Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23229 | 5' | -56.5 | NC_005259.1 | + | 26278 | 0.66 | 0.728793 |
Target: 5'- uGUCGCCgGUgccGCCCucgaCGGGuacGGGGAUCGa -3' miRNA: 3'- gUAGUGGgCG---UGGG----GCCU---UCUCUAGC- -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 7781 | 0.66 | 0.718463 |
Target: 5'- --cCGCCgaGCGCCCCGGcGGGGuuuUCu -3' miRNA: 3'- guaGUGGg-CGUGGGGCCuUCUCu--AGc -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 9817 | 0.66 | 0.708052 |
Target: 5'- --cCGCCCGCACCgCCGGGugcGGcgCu -3' miRNA: 3'- guaGUGGGCGUGG-GGCCUuc-UCuaGc -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 22215 | 0.67 | 0.63378 |
Target: 5'- --cCACCgCGCACCgCCGcGAGGcuGUCGg -3' miRNA: 3'- guaGUGG-GCGUGG-GGC-CUUCucUAGC- -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 55610 | 0.68 | 0.612403 |
Target: 5'- aCGUCuCgCGCGCCuuGGccGGGAUCa -3' miRNA: 3'- -GUAGuGgGCGUGGggCCuuCUCUAGc -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 18973 | 0.68 | 0.612403 |
Target: 5'- -cUCGCCgCGUACaucgCCCGGcacGAGAUCGg -3' miRNA: 3'- guAGUGG-GCGUG----GGGCCuu-CUCUAGC- -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 7027 | 0.68 | 0.601735 |
Target: 5'- --cCGCCgaGCGCCCCGGcGGGGuuuuUCGu -3' miRNA: 3'- guaGUGGg-CGUGGGGCCuUCUCu---AGC- -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 24630 | 0.68 | 0.601735 |
Target: 5'- uCAUCGgcauCCCGCACCCCGacGAcGAGAc-- -3' miRNA: 3'- -GUAGU----GGGCGUGGGGC--CUuCUCUagc -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 38811 | 0.68 | 0.591091 |
Target: 5'- gAUCGCCCGCGCCCgCGaugaccGAG-UCGa -3' miRNA: 3'- gUAGUGGGCGUGGG-GCcuu---CUCuAGC- -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 30370 | 0.69 | 0.528176 |
Target: 5'- gAUCuGCCCGCugCCCGGc-GAGAa-- -3' miRNA: 3'- gUAG-UGGGCGugGGGCCuuCUCUagc -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 49017 | 0.7 | 0.497633 |
Target: 5'- aCGUCuuccuCCCcCACCCCGGucGAG-UCGg -3' miRNA: 3'- -GUAGu----GGGcGUGGGGCCuuCUCuAGC- -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 5407 | 0.7 | 0.497633 |
Target: 5'- uCAUCACCCGCGCUggCCGccacGucGAGGUCu -3' miRNA: 3'- -GUAGUGGGCGUGG--GGC----CuuCUCUAGc -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 49844 | 0.7 | 0.487627 |
Target: 5'- aGUCAUCCGCACCCgaGGAucguuGGUCa -3' miRNA: 3'- gUAGUGGGCGUGGGg-CCUucu--CUAGc -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 561 | 0.72 | 0.39349 |
Target: 5'- -cUgACCCGCACCaCGGcguGGGGGUCGa -3' miRNA: 3'- guAgUGGGCGUGGgGCCu--UCUCUAGC- -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 20171 | 0.72 | 0.367629 |
Target: 5'- aCcgCGCCCGcCGCCUCGGcugacGAGAUCGc -3' miRNA: 3'- -GuaGUGGGC-GUGGGGCCuu---CUCUAGC- -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 53219 | 0.76 | 0.203975 |
Target: 5'- gCGUCGCCCGCGCCCUGcuuGAGGUUc -3' miRNA: 3'- -GUAGUGGGCGUGGGGCcuuCUCUAGc -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 29265 | 0.77 | 0.179029 |
Target: 5'- cCAUCGUCCGCGCCgUGGAcGAGAUCGc -3' miRNA: 3'- -GUAGUGGGCGUGGgGCCUuCUCUAGC- -5' |
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23229 | 5' | -56.5 | NC_005259.1 | + | 48832 | 1.08 | 0.001368 |
Target: 5'- cCAUCACCCGCACCCCGGAAGAGAUCGc -3' miRNA: 3'- -GUAGUGGGCGUGGGGCCUUCUCUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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