Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23231 | 3' | -49.8 | NC_005259.1 | + | 44805 | 0.66 | 0.972821 |
Target: 5'- cCGGUGGCCUggcccUUGGuca----GCAGCu -3' miRNA: 3'- -GCUACCGGAa----AACCuuuagcgCGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 11311 | 0.66 | 0.972821 |
Target: 5'- uGA-GGCCgagacGGg---CGCGCAGCu -3' miRNA: 3'- gCUaCCGGaaaa-CCuuuaGCGCGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 26637 | 0.66 | 0.966316 |
Target: 5'- uCGAUGGCC---UGGcgugCGCuCGGCa -3' miRNA: 3'- -GCUACCGGaaaACCuuuaGCGcGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 40526 | 0.66 | 0.963057 |
Target: 5'- aCGAUGGUCUguagguucuugccgUGGuAGUCGaCGCGcGCc -3' miRNA: 3'- -GCUACCGGAaa------------ACCuUUAGC-GCGU-CG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 45170 | 0.67 | 0.954615 |
Target: 5'- --uUGGCCUUgUUGcgcgccGAGGUCaGCGCGGUg -3' miRNA: 3'- gcuACCGGAA-AAC------CUUUAG-CGCGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 45495 | 0.67 | 0.945442 |
Target: 5'- uGGUGGCCUUggucgcugUGGccacCGCGcCGGUg -3' miRNA: 3'- gCUACCGGAAa-------ACCuuuaGCGC-GUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 50280 | 0.68 | 0.923661 |
Target: 5'- uCGGUGGCg----GGAAcGUCGUGCAcGCu -3' miRNA: 3'- -GCUACCGgaaaaCCUU-UAGCGCGU-CG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 68682 | 0.68 | 0.916221 |
Target: 5'- gGAUGGCCUUcucgaugaucGAGG-CGgGCAGCg -3' miRNA: 3'- gCUACCGGAAaac-------CUUUaGCgCGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 26948 | 0.68 | 0.91103 |
Target: 5'- ----cGCCgcugcUGGAAcgUGCGCAGCg -3' miRNA: 3'- gcuacCGGaaa--ACCUUuaGCGCGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 32650 | 0.68 | 0.91103 |
Target: 5'- --uUGGCCggggUggGGAAAUCGU-CGGCa -3' miRNA: 3'- gcuACCGGa---AaaCCUUUAGCGcGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 58955 | 0.68 | 0.904282 |
Target: 5'- ---cGGCCU--UGGccagcAGGUCGgGCAGCu -3' miRNA: 3'- gcuaCCGGAaaACC-----UUUAGCgCGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 47671 | 0.69 | 0.900096 |
Target: 5'- uGAUGGCCUuggccaccuuuugcaUgcccggUUGGA---UGCGCGGCa -3' miRNA: 3'- gCUACCGGA---------------A------AACCUuuaGCGCGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 1888 | 0.69 | 0.889936 |
Target: 5'- ---aGGCCgcc--GAGAUCGCGCgAGCu -3' miRNA: 3'- gcuaCCGGaaaacCUUUAGCGCG-UCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 3076 | 0.69 | 0.866365 |
Target: 5'- cCGA-GGCCgacaaGAGAgcCGCGCAGCg -3' miRNA: 3'- -GCUaCCGGaaaacCUUUa-GCGCGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 14078 | 0.71 | 0.802821 |
Target: 5'- gCGGUGGCCU------AcgUGCGCAGCg -3' miRNA: 3'- -GCUACCGGAaaaccuUuaGCGCGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 68094 | 0.73 | 0.712658 |
Target: 5'- uCGAUGGCCg---GGGuaacgacguaguucgGGUCGagaGCAGCg -3' miRNA: 3'- -GCUACCGGaaaaCCU---------------UUAGCg--CGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 7871 | 0.73 | 0.663647 |
Target: 5'- cCGGUGGCCcgagugcUUGGAc-UCGgGCAGCc -3' miRNA: 3'- -GCUACCGGaa-----AACCUuuAGCgCGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 12015 | 0.74 | 0.607268 |
Target: 5'- uCGGUGGCCUUguacGGug--CGgGCGGCa -3' miRNA: 3'- -GCUACCGGAAaa--CCuuuaGCgCGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 62757 | 0.75 | 0.584802 |
Target: 5'- gCGGUGGUaCUcgUGGGccggaacGUCGCGCAGCu -3' miRNA: 3'- -GCUACCG-GAaaACCUu------UAGCGCGUCG- -5' |
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23231 | 3' | -49.8 | NC_005259.1 | + | 58448 | 0.75 | 0.584802 |
Target: 5'- uCGGUGGCCU--UGGuGAUCGCGgccacCAGUg -3' miRNA: 3'- -GCUACCGGAaaACCuUUAGCGC-----GUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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