Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23232 | 3' | -56.1 | NC_005259.1 | + | 28015 | 0.73 | 0.376119 |
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Target: 5'- -gGGUCGCCGGGGuUGcgCGCGUUggccaGCCa -3' miRNA: 3'- agUCGGUGGCUCC-ACuaGCGCAG-----UGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 40886 | 0.73 | 0.376119 |
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Target: 5'- cCGGCCACCGuGGcggGGUCggcaGCGUC-CCa -3' miRNA: 3'- aGUCGGUGGCuCCa--CUAG----CGCAGuGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 19717 | 0.77 | 0.193149 |
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Target: 5'- gCuGCCGCCGAGGUGAUCGaCGgggacuaguugacUUACCc -3' miRNA: 3'- aGuCGGUGGCUCCACUAGC-GC-------------AGUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 46321 | 0.78 | 0.183792 |
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Target: 5'- gCGGCC-CCGAGG--GUCGCGUCGCg -3' miRNA: 3'- aGUCGGuGGCUCCacUAGCGCAGUGg -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 58447 | 0.78 | 0.179029 |
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Target: 5'- aUCGGUgGCCuuGGUGAUCGCGgcCACCa -3' miRNA: 3'- -AGUCGgUGGcuCCACUAGCGCa-GUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 63179 | 0.81 | 0.104438 |
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Target: 5'- --cGCCGCCGAGGUGAUCGCaccccggucGUgCGCCu -3' miRNA: 3'- aguCGGUGGCUCCACUAGCG---------CA-GUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 62122 | 0.88 | 0.034882 |
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Target: 5'- gUCAGUCACCGAGGUGAUCGUcUCgACCa -3' miRNA: 3'- -AGUCGGUGGCUCCACUAGCGcAG-UGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 47468 | 1.13 | 0.000662 |
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Target: 5'- gUCAGCCACCGAGGUGAUCGCGUCACCg -3' miRNA: 3'- -AGUCGGUGGCUCCACUAGCGCAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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