Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23232 | 3' | -56.1 | NC_005259.1 | + | 46589 | 0.67 | 0.718463 |
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Target: 5'- --cGCCGcCCGAGGcGAgcagCGCGUCGu- -3' miRNA: 3'- aguCGGU-GGCUCCaCUa---GCGCAGUgg -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 25926 | 0.67 | 0.718463 |
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Target: 5'- --cGCCGCCGGuccGGUGAgCGCa-CGCCg -3' miRNA: 3'- aguCGGUGGCU---CCACUaGCGcaGUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 5553 | 0.67 | 0.718463 |
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Target: 5'- -aGGCCGCgUGGGGUGGUgacgGCGagACCg -3' miRNA: 3'- agUCGGUG-GCUCCACUAg---CGCagUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 11015 | 0.66 | 0.728793 |
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Target: 5'- cCGGaCgCGCCGAGGcagGAguUCGCcgggugGUCACCg -3' miRNA: 3'- aGUC-G-GUGGCUCCa--CU--AGCG------CAGUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 60929 | 0.66 | 0.739032 |
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Target: 5'- -gAGCUcgGCCuuGGUGAUCGUcugCACCa -3' miRNA: 3'- agUCGG--UGGcuCCACUAGCGca-GUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 45734 | 0.66 | 0.749169 |
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Target: 5'- gCA-CCACCGAGGc---CGCG-CACCg -3' miRNA: 3'- aGUcGGUGGCUCCacuaGCGCaGUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 6440 | 0.66 | 0.759194 |
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Target: 5'- gUCGG-CACCGucGcgcUGAUCggcaGCGUCGCCg -3' miRNA: 3'- -AGUCgGUGGCucC---ACUAG----CGCAGUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 24553 | 0.66 | 0.769097 |
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Target: 5'- cCAGaUCGgCGGGGUGuacUCGUGggCACCg -3' miRNA: 3'- aGUC-GGUgGCUCCACu--AGCGCa-GUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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