Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23232 | 3' | -56.1 | NC_005259.1 | + | 1906 | 0.7 | 0.50773 |
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Target: 5'- -gAGCUGCCGAGGaag-CGCGcCGCCc -3' miRNA: 3'- agUCGGUGGCUCCacuaGCGCaGUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 58665 | 0.7 | 0.50773 |
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Target: 5'- cCGGUgCACCGGGGUGcagcuuccAUCGCGgggucggcucgUCGCCg -3' miRNA: 3'- aGUCG-GUGGCUCCAC--------UAGCGC-----------AGUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 39773 | 0.7 | 0.50773 |
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Target: 5'- gCAGCCugCugcuGGUGAUCG-GUgCGCCg -3' miRNA: 3'- aGUCGGugGcu--CCACUAGCgCA-GUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 16413 | 0.7 | 0.485637 |
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Target: 5'- gCAGcCCACCGAGGUGcccGUCgaGCGgcccaaaccgggCACCg -3' miRNA: 3'- aGUC-GGUGGCUCCAC---UAG--CGCa-----------GUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 6041 | 0.71 | 0.481669 |
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Target: 5'- uUCAGCgaCACCGAcgagcucgugugcgcGGUGAUCGC--CGCCg -3' miRNA: 3'- -AGUCG--GUGGCU---------------CCACUAGCGcaGUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 4476 | 0.71 | 0.477717 |
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Target: 5'- -aGGCCAUCGGcgguggaccauGGUGAUCGCcaccgCGCCg -3' miRNA: 3'- agUCGGUGGCU-----------CCACUAGCGca---GUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 21616 | 0.71 | 0.467908 |
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Target: 5'- aCA-CCACCGAGGcUGuGUCGCGcggaguguUCGCCg -3' miRNA: 3'- aGUcGGUGGCUCC-AC-UAGCGC--------AGUGG- -5' |
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| 23232 | 3' | -56.1 | NC_005259.1 | + | 47468 | 1.13 | 0.000662 |
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Target: 5'- gUCAGCCACCGAGGUGAUCGCGUCACCg -3' miRNA: 3'- -AGUCGGUGGCUCCACUAGCGCAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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