Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23234 | 5' | -49.8 | NC_005259.1 | + | 13414 | 0.66 | 0.969693 |
Target: 5'- --cUUCCUggccggGGUCGccgccgGGGCCGCGcuagGCGu -3' miRNA: 3'- cuaAAGGAa-----CUAGCa-----CCCGGUGU----UGC- -5' |
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23234 | 5' | -49.8 | NC_005259.1 | + | 43908 | 0.67 | 0.954615 |
Target: 5'- ----gCCggcUGAUCGaUGGGCaGCGACGa -3' miRNA: 3'- cuaaaGGa--ACUAGC-ACCCGgUGUUGC- -5' |
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23234 | 5' | -49.8 | NC_005259.1 | + | 16216 | 0.67 | 0.950169 |
Target: 5'- --gUUCC-UGAUCagGUGGGCCG-AACGc -3' miRNA: 3'- cuaAAGGaACUAG--CACCCGGUgUUGC- -5' |
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23234 | 5' | -49.8 | NC_005259.1 | + | 49855 | 0.67 | 0.950169 |
Target: 5'- ----cCCgagGAUCGUuGGUCACGACGc -3' miRNA: 3'- cuaaaGGaa-CUAGCAcCCGGUGUUGC- -5' |
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23234 | 5' | -49.8 | NC_005259.1 | + | 7926 | 0.68 | 0.923661 |
Target: 5'- ----cCCUUGGucUCGUGGGCCuucuuGCGc -3' miRNA: 3'- cuaaaGGAACU--AGCACCCGGugu--UGC- -5' |
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23234 | 5' | -49.8 | NC_005259.1 | + | 2178 | 0.69 | 0.866365 |
Target: 5'- cGGUUgCCgaGGUCaUGGGCCGCuACGg -3' miRNA: 3'- -CUAAaGGaaCUAGcACCCGGUGuUGC- -5' |
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23234 | 5' | -49.8 | NC_005259.1 | + | 45454 | 0.7 | 0.857986 |
Target: 5'- ---gUCCUUGAUCGgGGuGCCG-AACGg -3' miRNA: 3'- cuaaAGGAACUAGCaCC-CGGUgUUGC- -5' |
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23234 | 5' | -49.8 | NC_005259.1 | + | 5062 | 0.71 | 0.812548 |
Target: 5'- ----gUCgaGAUCGUGGGCCGgguCGACGg -3' miRNA: 3'- cuaaaGGaaCUAGCACCCGGU---GUUGC- -5' |
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23234 | 5' | -49.8 | NC_005259.1 | + | 45495 | 0.75 | 0.584802 |
Target: 5'- uGGUggCCUUGGUCGcUGuGGCCACcGCGc -3' miRNA: 3'- -CUAaaGGAACUAGC-AC-CCGGUGuUGC- -5' |
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23234 | 5' | -49.8 | NC_005259.1 | + | 17518 | 0.75 | 0.584802 |
Target: 5'- ---aUCCgaGcUCGUGGGCaCACAGCGa -3' miRNA: 3'- cuaaAGGaaCuAGCACCCG-GUGUUGC- -5' |
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23234 | 5' | -49.8 | NC_005259.1 | + | 47131 | 1.09 | 0.004761 |
Target: 5'- uGAUUUCCUUGAUCGUGGGCCACAACGu -3' miRNA: 3'- -CUAAAGGAACUAGCACCCGGUGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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