Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23235 | 5' | -55.6 | NC_005259.1 | + | 46404 | 1.12 | 0.000727 |
Target: 5'- gGUGAGGGCAUCGAAACCGGGCUGUGCa -3' miRNA: 3'- -CACUCCCGUAGCUUUGGCCCGACACG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 45852 | 0.66 | 0.771936 |
Target: 5'- --cGGGGCcggUGGGACCGGGCa-UGCc -3' miRNA: 3'- cacUCCCGua-GCUUUGGCCCGacACG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 60762 | 0.66 | 0.771936 |
Target: 5'- -aGucGGCucggCGAGGuuGGGCUGcGCg -3' miRNA: 3'- caCucCCGua--GCUUUggCCCGACaCG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 54491 | 0.66 | 0.761915 |
Target: 5'- -cGAGGGuCAUCGG---CGGGCaUGgcgGCg -3' miRNA: 3'- caCUCCC-GUAGCUuugGCCCG-ACa--CG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 8960 | 0.66 | 0.73112 |
Target: 5'- cGUGGacGGGCAcCGu-GCCGGGaCguagGUGCc -3' miRNA: 3'- -CACU--CCCGUaGCuuUGGCCC-Ga---CACG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 7174 | 0.67 | 0.714322 |
Target: 5'- -cGAGGGCAccggCGAgugcccggccugcggAcgcACCGGGCUGcgGUg -3' miRNA: 3'- caCUCCCGUa---GCU---------------U---UGGCCCGACa-CG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 7336 | 0.67 | 0.699456 |
Target: 5'- -cGAGuaCGUCGAGGCCGGacagacguucguGCUGUGg -3' miRNA: 3'- caCUCccGUAGCUUUGGCC------------CGACACg -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 10183 | 0.67 | 0.688759 |
Target: 5'- cUGAGGGCAgcacaUCGugaugacGACCGGGCa--GCu -3' miRNA: 3'- cACUCCCGU-----AGCu------UUGGCCCGacaCG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 56063 | 0.67 | 0.67801 |
Target: 5'- uUGAuGGCGUCGAGACCGucgaGCagaucgGUGCu -3' miRNA: 3'- cACUcCCGUAGCUUUGGCc---CGa-----CACG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 28867 | 0.67 | 0.667218 |
Target: 5'- -cGAGGGaCGUUG-GGCCGGGUacGUGUu -3' miRNA: 3'- caCUCCC-GUAGCuUUGGCCCGa-CACG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 25504 | 0.67 | 0.656394 |
Target: 5'- cGUGAGGGCAaaaaggcccUCGA--CCGuGGCaucgcguggGUGCu -3' miRNA: 3'- -CACUCCCGU---------AGCUuuGGC-CCGa--------CACG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 51528 | 0.67 | 0.656394 |
Target: 5'- cGUGAccucGuGCA-CGggGCCGGGCaaGUGCa -3' miRNA: 3'- -CACUc---C-CGUaGCuuUGGCCCGa-CACG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 65582 | 0.68 | 0.623831 |
Target: 5'- -gGAcGGGCGUC-AAACCGGGCUu--- -3' miRNA: 3'- caCU-CCCGUAGcUUUGGCCCGAcacg -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 30427 | 0.68 | 0.623831 |
Target: 5'- cGUGA-GGCAgcgCGAugAGCCGuGGCUGUucaGCu -3' miRNA: 3'- -CACUcCCGUa--GCU--UUGGC-CCGACA---CG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 23293 | 0.69 | 0.569815 |
Target: 5'- gGUGGuuGGGUAUCGggGuCCGGcGCggucgGUGUc -3' miRNA: 3'- -CACU--CCCGUAGCuuU-GGCC-CGa----CACG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 18842 | 0.69 | 0.537932 |
Target: 5'- gGUGAGGGUcUC---GCCGGuGCcGUGCa -3' miRNA: 3'- -CACUCCCGuAGcuuUGGCC-CGaCACG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 18127 | 0.7 | 0.500526 |
Target: 5'- -cGAGGGCAacaUCGGcaaggccauggacgaGAUCGGGCUGacGCc -3' miRNA: 3'- caCUCCCGU---AGCU---------------UUGGCCCGACa-CG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 32514 | 0.71 | 0.476237 |
Target: 5'- cGUGAGGuaGUCGAGG-CGGGCg--GCa -3' miRNA: 3'- -CACUCCcgUAGCUUUgGCCCGacaCG- -5' |
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23235 | 5' | -55.6 | NC_005259.1 | + | 48189 | 0.66 | 0.771936 |
Target: 5'- ---cGGGUGUCG-GACCGGGCa-UGCc -3' miRNA: 3'- cacuCCCGUAGCuUUGGCCCGacACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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