Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23236 | 3' | -56.1 | NC_005259.1 | + | 27399 | 0.71 | 0.44996 |
Target: 5'- ---gUGuCGgCAGCGgCGGCAGCUCGGu -3' miRNA: 3'- cuagAC-GUgGUCGUgGCUGUCGAGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 57357 | 0.66 | 0.722797 |
Target: 5'- aGAUgaGagaACgGGCACCGugGGCagucUCGGg -3' miRNA: 3'- -CUAgaCg--UGgUCGUGGCugUCG----AGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 35447 | 0.68 | 0.604949 |
Target: 5'- uAUCUGUccgcgcccACCGGCACCGcCAGCaccgccCGGu -3' miRNA: 3'- cUAGACG--------UGGUCGUGGCuGUCGa-----GCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 61994 | 0.68 | 0.594184 |
Target: 5'- -cUCUGCACCaugGGCACCGuGCGguguacccGCUCGc -3' miRNA: 3'- cuAGACGUGG---UCGUGGC-UGU--------CGAGCc -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 16365 | 0.68 | 0.594184 |
Target: 5'- aGAUCgUGCguGCCGcuGCGCCGACgAGCUCc- -3' miRNA: 3'- -CUAG-ACG--UGGU--CGUGGCUG-UCGAGcc -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 33254 | 0.68 | 0.58345 |
Target: 5'- aGAUCUGCcCgCAGCGCCGAU-GCUgCGc -3' miRNA: 3'- -CUAGACGuG-GUCGUGGCUGuCGA-GCc -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 3118 | 0.69 | 0.54098 |
Target: 5'- --aUUGCG-CGGCACCGugGGCagCGGa -3' miRNA: 3'- cuaGACGUgGUCGUGGCugUCGa-GCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 21995 | 0.7 | 0.509817 |
Target: 5'- ----gGCACCGGCAagUCGACGGUcaUCGGu -3' miRNA: 3'- cuagaCGUGGUCGU--GGCUGUCG--AGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 8503 | 0.7 | 0.509817 |
Target: 5'- uGGUgUGCACCAGaCGCagcgagcaCGACAGCcaaUCGGc -3' miRNA: 3'- -CUAgACGUGGUC-GUG--------GCUGUCG---AGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 63697 | 0.7 | 0.479432 |
Target: 5'- gGAUCUGCGCCuGC-CCaGCGGggaUCGGg -3' miRNA: 3'- -CUAGACGUGGuCGuGGcUGUCg--AGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 58398 | 0.71 | 0.453832 |
Target: 5'- ----cGCACCAGcCACCGGCGGCgaucagagauccacCGGg -3' miRNA: 3'- cuagaCGUGGUC-GUGGCUGUCGa-------------GCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 45591 | 1.09 | 0.000989 |
Target: 5'- gGAUCUGCACCAGCACCGACAGCUCGGu -3' miRNA: 3'- -CUAGACGUGGUCGUGGCUGUCGAGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 40157 | 0.71 | 0.412274 |
Target: 5'- uGUCgGUGCCcGCcCCGGCAGCUCGa -3' miRNA: 3'- cUAGaCGUGGuCGuGGCUGUCGAGCc -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 50412 | 0.72 | 0.394177 |
Target: 5'- ----cGCGCCGGUGCCuuGAcCGGCUCGGg -3' miRNA: 3'- cuagaCGUGGUCGUGG--CU-GUCGAGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 60947 | 0.72 | 0.385324 |
Target: 5'- cGUCUGCACCAcaugcGCGuCCGGCGGCagaUCGa -3' miRNA: 3'- cUAGACGUGGU-----CGU-GGCUGUCG---AGCc -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 32199 | 0.72 | 0.376603 |
Target: 5'- cGGUCUGCGacuuggccUCAGaCACCGACGcCUCGGc -3' miRNA: 3'- -CUAGACGU--------GGUC-GUGGCUGUcGAGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 46756 | 0.72 | 0.359565 |
Target: 5'- --gCUGCGCCGacaccuGCGCCGACAGC-CGc -3' miRNA: 3'- cuaGACGUGGU------CGUGGCUGUCGaGCc -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 5434 | 0.73 | 0.35125 |
Target: 5'- aGGUCUGCGCCcGCugccGCCGACAGaUCGc -3' miRNA: 3'- -CUAGACGUGGuCG----UGGCUGUCgAGCc -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 11992 | 0.74 | 0.275712 |
Target: 5'- --gCUGCACCAGCACauCGAUAGagUCGGu -3' miRNA: 3'- cuaGACGUGGUCGUG--GCUGUCg-AGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 58734 | 0.82 | 0.08476 |
Target: 5'- uGUCguugGCACCGGCACCGGCgaAGUUCGGc -3' miRNA: 3'- cUAGa---CGUGGUCGUGGCUG--UCGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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