Results 21 - 36 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23236 | 3' | -56.1 | NC_005259.1 | + | 52593 | 0.68 | 0.615737 |
Target: 5'- cGUCgccgaGCACCAGCGCCGuACGGUggccaacuacgUCGa -3' miRNA: 3'- cUAGa----CGUGGUCGUGGC-UGUCG-----------AGCc -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 56992 | 0.68 | 0.604949 |
Target: 5'- gGAUCUGagcuGCCcgugcgAGCACCucagcGCGGCUCGGc -3' miRNA: 3'- -CUAGACg---UGG------UCGUGGc----UGUCGAGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 35447 | 0.68 | 0.604949 |
Target: 5'- uAUCUGUccgcgcccACCGGCACCGcCAGCaccgccCGGu -3' miRNA: 3'- cUAGACG--------UGGUCGUGGCuGUCGa-----GCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 16365 | 0.68 | 0.594184 |
Target: 5'- aGAUCgUGCguGCCGcuGCGCCGACgAGCUCc- -3' miRNA: 3'- -CUAG-ACG--UGGU--CGUGGCUG-UCGAGcc -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 33254 | 0.68 | 0.58345 |
Target: 5'- aGAUCUGCcCgCAGCGCCGAU-GCUgCGc -3' miRNA: 3'- -CUAGACGuG-GUCGUGGCUGuCGA-GCc -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 15194 | 0.67 | 0.637345 |
Target: 5'- aAUCgUGCACCgcaugaugcuguGGCGCuCGGC-GCUCGGc -3' miRNA: 3'- cUAG-ACGUGG------------UCGUG-GCUGuCGAGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 2024 | 0.67 | 0.637345 |
Target: 5'- ----cGCACCGGCACCu-CAGUgCGGu -3' miRNA: 3'- cuagaCGUGGUCGUGGcuGUCGaGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 44716 | 0.67 | 0.637345 |
Target: 5'- cGAUCUGCAac-GCAcCCGGCAGCgCGc -3' miRNA: 3'- -CUAGACGUgguCGU-GGCUGUCGaGCc -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 19949 | 0.67 | 0.648147 |
Target: 5'- cGUgUGCGuCC-GCAaCGGCGGCUCGGc -3' miRNA: 3'- cUAgACGU-GGuCGUgGCUGUCGAGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 33612 | 0.67 | 0.657856 |
Target: 5'- cGAgCUGCAaCCGGcCACCGAaacuguugcgaauCGcGCUCGGg -3' miRNA: 3'- -CUaGACGU-GGUC-GUGGCU-------------GU-CGAGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 30124 | 0.67 | 0.691113 |
Target: 5'- --aUUGCcguCCGGCugCGAUAcCUCGGg -3' miRNA: 3'- cuaGACGu--GGUCGugGCUGUcGAGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 36862 | 0.67 | 0.695372 |
Target: 5'- --gCUGCGCCGuugcuggcaccgcccGCGCCGACGacGC-CGGu -3' miRNA: 3'- cuaGACGUGGU---------------CGUGGCUGU--CGaGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 58952 | 0.66 | 0.701743 |
Target: 5'- cGUCgGCcuugGCCAGCAgguCgGGCAGCUCGu -3' miRNA: 3'- cUAGaCG----UGGUCGU---GgCUGUCGAGCc -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 12141 | 0.66 | 0.710201 |
Target: 5'- gGGUCUGCuuucuugAUCAcCGCCGACGGCaccgcccUCGGu -3' miRNA: 3'- -CUAGACG-------UGGUcGUGGCUGUCG-------AGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 8470 | 0.66 | 0.722797 |
Target: 5'- aGAgCUGCACgAGCACCGcGCuG-UCGGc -3' miRNA: 3'- -CUaGACGUGgUCGUGGC-UGuCgAGCC- -5' |
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23236 | 3' | -56.1 | NC_005259.1 | + | 57357 | 0.66 | 0.722797 |
Target: 5'- aGAUgaGagaACgGGCACCGugGGCagucUCGGg -3' miRNA: 3'- -CUAgaCg--UGgUCGUGGCugUCG----AGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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