Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23237 | 3' | -56.4 | NC_005259.1 | + | 58654 | 0.66 | 0.726705 |
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Target: 5'- ----cUCGCGgu-AGCCCGGUGCaCCg -3' miRNA: 3'- gcucaAGCGCaacUCGGGCUACG-GGa -5' |
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| 23237 | 3' | -56.4 | NC_005259.1 | + | 42605 | 0.66 | 0.705829 |
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Target: 5'- uCGAcaUCgGCGgucaUGAGCCCGAUGUCg- -3' miRNA: 3'- -GCUcaAG-CGCa---ACUCGGGCUACGGga -5' |
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| 23237 | 3' | -56.4 | NC_005259.1 | + | 38924 | 0.68 | 0.620392 |
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Target: 5'- uGAGcacgUCgGUGUUGcuguGCCCGGUGCCgCUg -3' miRNA: 3'- gCUCa---AG-CGCAACu---CGGGCUACGG-GA- -5' |
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| 23237 | 3' | -56.4 | NC_005259.1 | + | 51334 | 0.68 | 0.588255 |
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Target: 5'- aCGAGgaUGCcggUGAGCUCGGauaUGCCCg -3' miRNA: 3'- -GCUCaaGCGca-ACUCGGGCU---ACGGGa -5' |
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| 23237 | 3' | -56.4 | NC_005259.1 | + | 50691 | 0.7 | 0.500567 |
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Target: 5'- aCGAGgcgcugcucgucgUCGCGcUUGAGCCgGGUGCUg- -3' miRNA: 3'- -GCUCa------------AGCGC-AACUCGGgCUACGGga -5' |
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| 23237 | 3' | -56.4 | NC_005259.1 | + | 48083 | 0.7 | 0.464729 |
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Target: 5'- uGAGcgaUgGUGUUGAGCCCGAcggGUCCg -3' miRNA: 3'- gCUCa--AgCGCAACUCGGGCUa--CGGGa -5' |
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| 23237 | 3' | -56.4 | NC_005259.1 | + | 45210 | 1.08 | 0.001205 |
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Target: 5'- gCGAGUUCGCGUUGAGCCCGAUGCCCUg -3' miRNA: 3'- -GCUCAAGCGCAACUCGGGCUACGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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