Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23243 | 3' | -60 | NC_005259.1 | + | 42412 | 1.04 | 0.000802 |
Target: 5'- gUACUCGAUGGCGGUGCGGCAGCCGAAa -3' miRNA: 3'- -AUGAGCUACCGCCACGCCGUCGGCUU- -5' |
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23243 | 3' | -60 | NC_005259.1 | + | 10330 | 0.66 | 0.490113 |
Target: 5'- gGCUCGgcGaCGGgcucgGCGGCAGCgGGc -3' miRNA: 3'- aUGAGCuaCcGCCa----CGCCGUCGgCUu -5' |
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23243 | 3' | -60 | NC_005259.1 | + | 61026 | 0.66 | 0.480151 |
Target: 5'- cACUCGGUGGCGGcGaGGUcGaCCGAc -3' miRNA: 3'- aUGAGCUACCGCCaCgCCGuC-GGCUu -5' |
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23243 | 3' | -60 | NC_005259.1 | + | 50430 | 0.66 | 0.460531 |
Target: 5'- gGCUCGGgcaGCGGUGCGGgCucggggucGCCGGu -3' miRNA: 3'- aUGAGCUac-CGCCACGCC-Gu-------CGGCUu -5' |
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23243 | 3' | -60 | NC_005259.1 | + | 49414 | 0.67 | 0.429116 |
Target: 5'- uUGCUCGAUgccagcgagaaaccGGCgagcgccgccuuGGU-CGGCAGCCGGAu -3' miRNA: 3'- -AUGAGCUA--------------CCG------------CCAcGCCGUCGGCUU- -5' |
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23243 | 3' | -60 | NC_005259.1 | + | 43726 | 0.67 | 0.422616 |
Target: 5'- gAgUCGAUGGCGGccaUGCcGCcgAGCCGGg -3' miRNA: 3'- aUgAGCUACCGCC---ACGcCG--UCGGCUu -5' |
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23243 | 3' | -60 | NC_005259.1 | + | 55010 | 0.67 | 0.404375 |
Target: 5'- gGCUCGcuUGGCGGUcGUcucggcacgGGCuGCCGAGg -3' miRNA: 3'- aUGAGCu-ACCGCCA-CG---------CCGuCGGCUU- -5' |
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23243 | 3' | -60 | NC_005259.1 | + | 19968 | 0.67 | 0.395443 |
Target: 5'- gGCUC---GGCGGUGUGGguGCCc-- -3' miRNA: 3'- aUGAGcuaCCGCCACGCCguCGGcuu -5' |
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23243 | 3' | -60 | NC_005259.1 | + | 60420 | 0.67 | 0.395443 |
Target: 5'- cUGCUCGggGGUGGcccacGgGGCGGCCa-- -3' miRNA: 3'- -AUGAGCuaCCGCCa----CgCCGUCGGcuu -5' |
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23243 | 3' | -60 | NC_005259.1 | + | 27388 | 0.68 | 0.352741 |
Target: 5'- gAC-CGgcGGCGGUGuCGGCAGCgGc- -3' miRNA: 3'- aUGaGCuaCCGCCAC-GCCGUCGgCuu -5' |
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23243 | 3' | -60 | NC_005259.1 | + | 42544 | 0.69 | 0.321001 |
Target: 5'- cACcgaGGUGGCGGgcagcucgGCGaGCAGCCGGu -3' miRNA: 3'- aUGag-CUACCGCCa-------CGC-CGUCGGCUu -5' |
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23243 | 3' | -60 | NC_005259.1 | + | 10453 | 0.72 | 0.210112 |
Target: 5'- gGCUCGAcGGCGGUGUccuGcGCAGCgGGAc -3' miRNA: 3'- aUGAGCUaCCGCCACG---C-CGUCGgCUU- -5' |
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23243 | 3' | -60 | NC_005259.1 | + | 27985 | 0.72 | 0.199491 |
Target: 5'- -cCUCccuugGGUGGUGCGGguGCCGGGg -3' miRNA: 3'- auGAGcua--CCGCCACGCCguCGGCUU- -5' |
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23243 | 3' | -60 | NC_005259.1 | + | 67158 | 0.66 | 0.500171 |
Target: 5'- cUugUCGAUGGUGGcgGCcauGCcGCCGAu -3' miRNA: 3'- -AugAGCUACCGCCa-CGc--CGuCGGCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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