Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23248 | 5' | -58.1 | NC_005259.1 | + | 50485 | 0.67 | 0.515556 |
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Target: 5'- -aACCCUGCUCgaugAGCCggUCGaugUGGGCGg -3' miRNA: 3'- uaUGGGACGGG----UCGG--AGCa--ACUCGCg -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 52392 | 0.7 | 0.38171 |
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Target: 5'- -cAgCCUGCCUcgGGCCaUCGaggugUGGGCGCu -3' miRNA: 3'- uaUgGGACGGG--UCGG-AGCa----ACUCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 54536 | 0.67 | 0.515556 |
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Target: 5'- aGUAUCCgGCCCAGCCacacaGaUUGAacuuGCGCg -3' miRNA: 3'- -UAUGGGaCGGGUCGGag---C-AACU----CGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 55525 | 0.72 | 0.279266 |
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Target: 5'- -aACUgUGCCCAcagcgccGCCUCGUUG-GCGUc -3' miRNA: 3'- uaUGGgACGGGU-------CGGAGCAACuCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 58642 | 0.66 | 0.566863 |
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Target: 5'- aGUGCCCaUGCCCucgcgguAGCC-CGgugcaccgGGGUGCa -3' miRNA: 3'- -UAUGGG-ACGGG-------UCGGaGCaa------CUCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 63142 | 0.67 | 0.536298 |
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Target: 5'- -aGCCgCUGUCCucGCCcaCGUUGAGCaGCc -3' miRNA: 3'- uaUGG-GACGGGu-CGGa-GCAACUCG-CG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 63701 | 0.72 | 0.279959 |
|
Target: 5'- cUGCgCCUGCCCAGCggggaUCGggGccuGCGCa -3' miRNA: 3'- uAUG-GGACGGGUCGg----AGCaaCu--CGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 64748 | 0.67 | 0.525889 |
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Target: 5'- -cACCC-GCCCAccgcGCCUCGcgacgGuGCGCc -3' miRNA: 3'- uaUGGGaCGGGU----CGGAGCaa---CuCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 64799 | 0.67 | 0.515556 |
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Target: 5'- -gGCUgugUGCCuCGGCCUCGgcggcaaUGAGCGUg -3' miRNA: 3'- uaUGGg--ACGG-GUCGGAGCa------ACUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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