Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23248 | 5' | -58.1 | NC_005259.1 | + | 52392 | 0.7 | 0.38171 |
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Target: 5'- -cAgCCUGCCUcgGGCCaUCGaggugUGGGCGCu -3' miRNA: 3'- uaUgGGACGGG--UCGG-AGCa----ACUCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 33977 | 0.69 | 0.408441 |
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Target: 5'- -cACCUUGCCCugAGCCggguaugCGgUGAGCuGCa -3' miRNA: 3'- uaUGGGACGGG--UCGGa------GCaACUCG-CG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 45162 | 0.68 | 0.47511 |
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Target: 5'- -gGCC--GCCCuuGGCCUUGUUGcGCGCc -3' miRNA: 3'- uaUGGgaCGGG--UCGGAGCAACuCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 32544 | 0.68 | 0.485079 |
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Target: 5'- -gACCUUGCUguUGGCCgcccCGgcGAGCGCg -3' miRNA: 3'- uaUGGGACGG--GUCGGa---GCaaCUCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 36727 | 0.67 | 0.509396 |
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Target: 5'- -gACCCgccgcGCCC-GCCgggauacgugauccCGUUGAGCGUg -3' miRNA: 3'- uaUGGGa----CGGGuCGGa-------------GCAACUCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 9543 | 0.67 | 0.515556 |
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Target: 5'- -cACCggagagCUGCCCGGCCUCGacc-GCGUc -3' miRNA: 3'- uaUGG------GACGGGUCGGAGCaacuCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 50485 | 0.67 | 0.515556 |
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Target: 5'- -aACCCUGCUCgaugAGCCggUCGaugUGGGCGg -3' miRNA: 3'- uaUGGGACGGG----UCGG--AGCa--ACUCGCg -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 54536 | 0.67 | 0.515556 |
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Target: 5'- aGUAUCCgGCCCAGCCacacaGaUUGAacuuGCGCg -3' miRNA: 3'- -UAUGGGaCGGGUCGGag---C-AACU----CGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 43900 | 0.66 | 0.610757 |
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Target: 5'- uUGCCC-GCgCCGGCUgaUCGaUGGGCaGCg -3' miRNA: 3'- uAUGGGaCG-GGUCGG--AGCaACUCG-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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