Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23249 | 3' | -49.2 | NC_005259.1 | + | 63336 | 0.66 | 0.983629 |
Target: 5'- gCCugGcAGAUCAGGUccaUGaCGGGCuuGa -3' miRNA: 3'- -GGugU-UCUAGUUCAa--ACaGUCCGggC- -5' |
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23249 | 3' | -49.2 | NC_005259.1 | + | 19156 | 0.66 | 0.983629 |
Target: 5'- aCCGauCAGGGUCGAGguucGUCGGcGCUCa -3' miRNA: 3'- -GGU--GUUCUAGUUCaaa-CAGUC-CGGGc -5' |
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23249 | 3' | -49.2 | NC_005259.1 | + | 5237 | 0.66 | 0.981467 |
Target: 5'- aCGCuGAGAUCGA---UGUguGGCUCGg -3' miRNA: 3'- gGUG-UUCUAGUUcaaACAguCCGGGC- -5' |
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23249 | 3' | -49.2 | NC_005259.1 | + | 32764 | 0.66 | 0.975411 |
Target: 5'- aCCGCGaacAGAUCGAGUUgcguggcacccgaGUCGuaGCCCGa -3' miRNA: 3'- -GGUGU---UCUAGUUCAAa------------CAGUc-CGGGC- -5' |
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23249 | 3' | -49.2 | NC_005259.1 | + | 68676 | 0.67 | 0.970643 |
Target: 5'- gCCACGGGAuggccuucucgaugaUCGAGgcgggcagcgcGUCGGuGCCCGa -3' miRNA: 3'- -GGUGUUCU---------------AGUUCaaa--------CAGUC-CGGGC- -5' |
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23249 | 3' | -49.2 | NC_005259.1 | + | 4215 | 0.67 | 0.9623 |
Target: 5'- cUCAuCGAGAUCGAGcaggccaagCAGGCCCc -3' miRNA: 3'- -GGU-GUUCUAGUUCaaaca----GUCCGGGc -5' |
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23249 | 3' | -49.2 | NC_005259.1 | + | 49476 | 0.67 | 0.955899 |
Target: 5'- gUCGCccuuGAacUCAAGUUUGUCguAGGUCCa -3' miRNA: 3'- -GGUGuu--CU--AGUUCAAACAG--UCCGGGc -5' |
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23249 | 3' | -49.2 | NC_005259.1 | + | 58337 | 0.68 | 0.951544 |
Target: 5'- -aGCGAGAUCAGGUcgGU--GGCCuCGg -3' miRNA: 3'- ggUGUUCUAGUUCAaaCAguCCGG-GC- -5' |
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23249 | 3' | -49.2 | NC_005259.1 | + | 834 | 0.69 | 0.925516 |
Target: 5'- aCCACGAGAcCAAGgucgcUGcgCAGGCCg- -3' miRNA: 3'- -GGUGUUCUaGUUCaa---ACa-GUCCGGgc -5' |
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23249 | 3' | -49.2 | NC_005259.1 | + | 49075 | 0.7 | 0.892288 |
Target: 5'- gCGCAAGGgaucgcUCGAGggUGUCAuGGCCg- -3' miRNA: 3'- gGUGUUCU------AGUUCaaACAGU-CCGGgc -5' |
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23249 | 3' | -49.2 | NC_005259.1 | + | 23194 | 0.73 | 0.755201 |
Target: 5'- -gACGAGAUCGuGUUcGUguGGCCCa -3' miRNA: 3'- ggUGUUCUAGUuCAAaCAguCCGGGc -5' |
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23249 | 3' | -49.2 | NC_005259.1 | + | 10396 | 0.73 | 0.744583 |
Target: 5'- cCCGCAGGAUCucgc---UCAGGCCCu -3' miRNA: 3'- -GGUGUUCUAGuucaaacAGUCCGGGc -5' |
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23249 | 3' | -49.2 | NC_005259.1 | + | 38007 | 1.11 | 0.004426 |
Target: 5'- uCCACAAGAUCAAGUUUGUCAGGCCCGc -3' miRNA: 3'- -GGUGUUCUAGUUCAAACAGUCCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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