Results 101 - 104 of 104 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23249 | 5' | -61.5 | NC_005259.1 | + | 67983 | 0.69 | 0.306792 |
Target: 5'- gGCgUGGCCACCGCcgcgagcgucgacuuGCCC--UCCUGCg -3' miRNA: 3'- -UGgGCCGGUGGUG---------------UGGGcuAGGACGg -5' |
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23249 | 5' | -61.5 | NC_005259.1 | + | 4358 | 0.69 | 0.311113 |
Target: 5'- aGCgCCGGaCGgCACGCCCGugcCCUGCUc -3' miRNA: 3'- -UG-GGCCgGUgGUGUGGGCua-GGACGG- -5' |
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23249 | 5' | -61.5 | NC_005259.1 | + | 63674 | 0.69 | 0.318415 |
Target: 5'- cACCuCGGgCACCgGCGCgCCGGggaucugcgCCUGCCc -3' miRNA: 3'- -UGG-GCCgGUGG-UGUG-GGCUa--------GGACGG- -5' |
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23249 | 5' | -61.5 | NC_005259.1 | + | 32990 | 0.68 | 0.364912 |
Target: 5'- -gCCGaggguGCCGCCACGCCCGAUgUCgGCa -3' miRNA: 3'- ugGGC-----CGGUGGUGUGGGCUA-GGaCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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