miRNA display CGI


Results 21 - 26 of 26 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23253 5' -57.1 NC_005259.1 + 38869 0.66 0.650718
Target:  5'- --cGACgCGCCCGCaaugGCGGUGUugacGACGCu -3'
miRNA:   3'- aacUUG-GUGGGCG----CGCCACA----CUGUGc -5'
23253 5' -57.1 NC_005259.1 + 30019 0.66 0.661448
Target:  5'- gUGAccACCGCCgGgGCGGUGgGACu-- -3'
miRNA:   3'- aACU--UGGUGGgCgCGCCACaCUGugc -5'
23253 5' -57.1 NC_005259.1 + 16104 0.66 0.682819
Target:  5'- -aGAugUACCgGCGCGGaaUGAC-CGa -3'
miRNA:   3'- aaCUugGUGGgCGCGCCacACUGuGC- -5'
23253 5' -57.1 NC_005259.1 + 36874 0.66 0.682819
Target:  5'- cUGGcACCGCCCGCGCcgacgacgccGGUGagcgccccacUGACGgGg -3'
miRNA:   3'- aACU-UGGUGGGCGCG----------CCAC----------ACUGUgC- -5'
23253 5' -57.1 NC_005259.1 + 18727 0.66 0.682819
Target:  5'- -cGGGCCGCCCGgcaucgguaaguCGuCGGUGgugcucGACAUGg -3'
miRNA:   3'- aaCUUGGUGGGC------------GC-GCCACa-----CUGUGC- -5'
23253 5' -57.1 NC_005259.1 + 39402 0.66 0.69344
Target:  5'- cUUGGuCCAcCCCGagGCGGUGccgGACACc -3'
miRNA:   3'- -AACUuGGU-GGGCg-CGCCACa--CUGUGc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.