Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
23253 | 5' | -57.1 | NC_005259.1 | + | 38869 | 0.66 | 0.650718 |
Target: 5'- --cGACgCGCCCGCaaugGCGGUGUugacGACGCu -3' miRNA: 3'- aacUUG-GUGGGCG----CGCCACA----CUGUGc -5' |
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23253 | 5' | -57.1 | NC_005259.1 | + | 30019 | 0.66 | 0.661448 |
Target: 5'- gUGAccACCGCCgGgGCGGUGgGACu-- -3' miRNA: 3'- aACU--UGGUGGgCgCGCCACaCUGugc -5' |
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23253 | 5' | -57.1 | NC_005259.1 | + | 16104 | 0.66 | 0.682819 |
Target: 5'- -aGAugUACCgGCGCGGaaUGAC-CGa -3' miRNA: 3'- aaCUugGUGGgCGCGCCacACUGuGC- -5' |
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23253 | 5' | -57.1 | NC_005259.1 | + | 36874 | 0.66 | 0.682819 |
Target: 5'- cUGGcACCGCCCGCGCcgacgacgccGGUGagcgccccacUGACGgGg -3' miRNA: 3'- aACU-UGGUGGGCGCG----------CCAC----------ACUGUgC- -5' |
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23253 | 5' | -57.1 | NC_005259.1 | + | 18727 | 0.66 | 0.682819 |
Target: 5'- -cGGGCCGCCCGgcaucgguaaguCGuCGGUGgugcucGACAUGg -3' miRNA: 3'- aaCUUGGUGGGC------------GC-GCCACa-----CUGUGC- -5' |
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23253 | 5' | -57.1 | NC_005259.1 | + | 39402 | 0.66 | 0.69344 |
Target: 5'- cUUGGuCCAcCCCGagGCGGUGccgGACACc -3' miRNA: 3'- -AACUuGGU-GGGCg-CGCCACa--CUGUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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