Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23253 | 5' | -57.1 | NC_005259.1 | + | 19104 | 0.71 | 0.378964 |
Target: 5'- -cGAACuCACCgGCGCuGGUGUGugcgucgugcaccACACGa -3' miRNA: 3'- aaCUUG-GUGGgCGCG-CCACAC-------------UGUGC- -5' |
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23253 | 5' | -57.1 | NC_005259.1 | + | 36046 | 0.7 | 0.414595 |
Target: 5'- -cGAgGCCACCCGCGCGagcaccaccugcuGUGUGcucguCGCGg -3' miRNA: 3'- aaCU-UGGUGGGCGCGC-------------CACACu----GUGC- -5' |
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23253 | 5' | -57.1 | NC_005259.1 | + | 53184 | 0.69 | 0.502729 |
Target: 5'- ---cGCCGCCCGuCGCGGUGUccccGGCGa- -3' miRNA: 3'- aacuUGGUGGGC-GCGCCACA----CUGUgc -5' |
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23253 | 5' | -57.1 | NC_005259.1 | + | 19841 | 0.69 | 0.512927 |
Target: 5'- -cGGcuGCUGCCgCGCuCGGUGUGGCGCu -3' miRNA: 3'- aaCU--UGGUGG-GCGcGCCACACUGUGc -5' |
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23253 | 5' | -57.1 | NC_005259.1 | + | 37744 | 0.68 | 0.554502 |
Target: 5'- -cGAGCUGCUCgaugaccaGCGCGGUGgccccugcgGGCACGa -3' miRNA: 3'- aaCUUGGUGGG--------CGCGCCACa--------CUGUGC- -5' |
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23253 | 5' | -57.1 | NC_005259.1 | + | 39402 | 0.66 | 0.69344 |
Target: 5'- cUUGGuCCAcCCCGagGCGGUGccgGACACc -3' miRNA: 3'- -AACUuGGU-GGGCg-CGCCACa--CUGUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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