miRNA display CGI


Results 21 - 23 of 23 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23254 5' -57.2 NC_005259.1 + 58583 0.69 0.442185
Target:  5'- gCGGCGCgGCuGCCGGGcCGGAUcuuuucGAGCGc -3'
miRNA:   3'- -GUCGCGgUG-CGGCCU-GUCUAa-----CUCGU- -5'
23254 5' -57.2 NC_005259.1 + 66256 0.69 0.491358
Target:  5'- gCAGCGCggccCGCGCCGGGCGGc---GGCu -3'
miRNA:   3'- -GUCGCG----GUGCGGCCUGUCuaacUCGu -5'
23254 5' -57.2 NC_005259.1 + 68539 0.68 0.532444
Target:  5'- --cCGCCACGCgucgacauCGGGguGGUUGAGCc -3'
miRNA:   3'- gucGCGGUGCG--------GCCUguCUAACUCGu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.