Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23255 | 3' | -61.3 | NC_005259.1 | + | 42945 | 0.73 | 0.202034 |
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Target: 5'- gCCGugGGCGAGGCCGccgUUGUcgaacgggauGCCGGg -3' miRNA: 3'- -GGCugCCGCUCCGGCcu-GACG----------UGGCU- -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 34568 | 0.74 | 0.152977 |
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Target: 5'- gCCGACGGCGAGGUucuCGGACagGCAgacgcgcggugUCGAc -3' miRNA: 3'- -GGCUGCCGCUCCG---GCCUGa-CGU-----------GGCU- -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 60763 | 0.77 | 0.106248 |
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Target: 5'- gUCGGCucGGCGAGGuuGGGCUGCG-CGAa -3' miRNA: 3'- -GGCUG--CCGCUCCggCCUGACGUgGCU- -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 7830 | 0.77 | 0.093076 |
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Target: 5'- cCCGGCGGCGgucaccccuuacGGGaCGGACcGCGCCGAc -3' miRNA: 3'- -GGCUGCCGC------------UCCgGCCUGaCGUGGCU- -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 8330 | 0.78 | 0.083668 |
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Target: 5'- gCCGACGGCGAucaGCCGGucacGCUGCuGCCGGu -3' miRNA: 3'- -GGCUGCCGCUc--CGGCC----UGACG-UGGCU- -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 4783 | 0.81 | 0.052916 |
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Target: 5'- uCCGcaACGGCGAGGCCaGGuACUGC-CCGAg -3' miRNA: 3'- -GGC--UGCCGCUCCGG-CC-UGACGuGGCU- -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 23655 | 0.82 | 0.044943 |
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Target: 5'- gCCGACGGCGAcGGCUGGAC--CGCCGAc -3' miRNA: 3'- -GGCUGCCGCU-CCGGCCUGacGUGGCU- -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 35992 | 1.1 | 0.000358 |
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Target: 5'- aCCGACGGCGAGGCCGGACUGCACCGAg -3' miRNA: 3'- -GGCUGCCGCUCCGGCCUGACGUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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