Results 61 - 68 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23255 | 3' | -61.3 | NC_005259.1 | + | 13454 | 0.66 | 0.498029 |
Target: 5'- aCCGuCGGCGcGGcGCUGGugcucaccgcGCUGC-CCGAc -3' miRNA: 3'- -GGCuGCCGC-UC-CGGCC----------UGACGuGGCU- -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 14650 | 0.66 | 0.498029 |
Target: 5'- aCGAUGGCGuGGGCucagcccgacuCGGGCcgcgUGCACuCGAu -3' miRNA: 3'- gGCUGCCGC-UCCG-----------GCCUG----ACGUG-GCU- -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 47605 | 0.66 | 0.498029 |
Target: 5'- uCUGGuCGGCGAGGCUGaGGuucUUGUcgucGCCGAg -3' miRNA: 3'- -GGCU-GCCGCUCCGGC-CU---GACG----UGGCU- -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 19249 | 0.66 | 0.488415 |
Target: 5'- aUCGAUGGCcgccuGCCGGGCcGC-CCGAu -3' miRNA: 3'- -GGCUGCCGcuc--CGGCCUGaCGuGGCU- -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 60344 | 0.66 | 0.488415 |
Target: 5'- -aGGCGGCGAucaGGUCGaGGCccUGC-CCGAa -3' miRNA: 3'- ggCUGCCGCU---CCGGC-CUG--ACGuGGCU- -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 11775 | 0.66 | 0.488415 |
Target: 5'- cUCGACGGCGAGGgagucccacacaUCGGGC-GCgACCc- -3' miRNA: 3'- -GGCUGCCGCUCC------------GGCCUGaCG-UGGcu -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 44870 | 0.66 | 0.47889 |
Target: 5'- aCCGGCGGCGAGaUCcGAC-GCGCCc- -3' miRNA: 3'- -GGCUGCCGCUCcGGcCUGaCGUGGcu -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 37499 | 0.66 | 0.527352 |
Target: 5'- cCCGGCGGUGA-GCaCGauGCUGC-CCGAc -3' miRNA: 3'- -GGCUGCCGCUcCG-GCc-UGACGuGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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