Results 61 - 68 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23255 | 3' | -61.3 | NC_005259.1 | + | 7427 | 0.66 | 0.517502 |
Target: 5'- gUCGGCGGUGcccGcGCCGcauGGCaGCGCCGAg -3' miRNA: 3'- -GGCUGCCGCu--C-CGGC---CUGaCGUGGCU- -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 7334 | 0.68 | 0.406291 |
Target: 5'- gCCGAguaCGuCGAGGCCGGACagacguucgUGCugUGGc -3' miRNA: 3'- -GGCU---GCcGCUCCGGCCUG---------ACGugGCU- -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 7181 | 0.69 | 0.333395 |
Target: 5'- aCCGGCGaGUGcccGGCCugcGGAC-GCACCGGg -3' miRNA: 3'- -GGCUGC-CGCu--CCGG---CCUGaCGUGGCU- -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 5068 | 0.69 | 0.348736 |
Target: 5'- aUCGugGGCcgggucgacGGGCCGGugU-CACCGGc -3' miRNA: 3'- -GGCugCCGc--------UCCGGCCugAcGUGGCU- -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 4783 | 0.81 | 0.052916 |
Target: 5'- uCCGcaACGGCGAGGCCaGGuACUGC-CCGAg -3' miRNA: 3'- -GGC--UGCCGCUCCGG-CC-UGACGuGGCU- -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 4257 | 0.68 | 0.372676 |
Target: 5'- cUCGACuGCGAGGacaaCGGgcgGCUGCGCUGc -3' miRNA: 3'- -GGCUGcCGCUCCg---GCC---UGACGUGGCu -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 3298 | 0.66 | 0.527352 |
Target: 5'- gCCGGucccuCGGCGAGcGCUGcgucGACgugaGCGCCGGg -3' miRNA: 3'- -GGCU-----GCCGCUC-CGGC----CUGa---CGUGGCU- -5' |
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23255 | 3' | -61.3 | NC_005259.1 | + | 737 | 0.66 | 0.504808 |
Target: 5'- aCCGGCGGUGccuacgccGCCGGugccuaccgcguccACcGCGCCGAg -3' miRNA: 3'- -GGCUGCCGCuc------CGGCC--------------UGaCGUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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