Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23255 | 3' | -61.3 | NC_005259.1 | + | 41724 | 0.69 | 0.318555 |
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Target: 5'- aCCGGCGGCGGGGa-GGACaccGCcUCGAc -3' miRNA: 3'- -GGCUGCCGCUCCggCCUGa--CGuGGCU- -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 67044 | 0.69 | 0.318555 |
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Target: 5'- cUCGACGGCGAGcacCCG---UGCGCCGAu -3' miRNA: 3'- -GGCUGCCGCUCc--GGCcugACGUGGCU- -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 36929 | 0.69 | 0.318555 |
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Target: 5'- gCCGACGGUGAGuGUU--GCUGuCACCGGg -3' miRNA: 3'- -GGCUGCCGCUC-CGGccUGAC-GUGGCU- -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 16585 | 0.7 | 0.310607 |
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Target: 5'- gCCGAgGacaucgcccGCGAGGCCaucGGACUGUucaacggGCCGAg -3' miRNA: 3'- -GGCUgC---------CGCUCCGG---CCUGACG-------UGGCU- -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 60621 | 0.7 | 0.304217 |
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Target: 5'- -aGGCGGCGAGGUgUGGAaCUGCGCg-- -3' miRNA: 3'- ggCUGCCGCUCCG-GCCU-GACGUGgcu -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 46397 | 0.7 | 0.304217 |
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Target: 5'- gCCGcGCGGUGAGGgcaucgaaaCCGGGCugUGCACCc- -3' miRNA: 3'- -GGC-UGCCGCUCC---------GGCCUG--ACGUGGcu -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 61032 | 0.7 | 0.289701 |
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Target: 5'- gUGGCGGCGAGGUcgaccgaCGGGCggcucaccuUGUGCCGGg -3' miRNA: 3'- gGCUGCCGCUCCG-------GCCUG---------ACGUGGCU- -5' |
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| 23255 | 3' | -61.3 | NC_005259.1 | + | 35992 | 1.1 | 0.000358 |
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Target: 5'- aCCGACGGCGAGGCCGGACUGCACCGAg -3' miRNA: 3'- -GGCUGCCGCUCCGGCCUGACGUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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