Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23259 | 3' | -55.7 | NC_005259.1 | + | 52205 | 0.66 | 0.7254 |
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Target: 5'- aCGGCUcaUG-GGCaccgCGUUCCGCGcCCGCu -3' miRNA: 3'- -GUCGA--ACuCCGa---GUAGGGUGCuGGUG- -5' |
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| 23259 | 3' | -55.7 | NC_005259.1 | + | 14654 | 0.66 | 0.704141 |
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Target: 5'- uGGCgUG-GGCUCAgCCCGacucgGGCCGCg -3' miRNA: 3'- gUCGaACuCCGAGUaGGGUg----CUGGUG- -5' |
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| 23259 | 3' | -55.7 | NC_005259.1 | + | 36135 | 0.66 | 0.693402 |
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Target: 5'- gGGCcgccGGGCUgucCGUCgCCAUGGCCACg -3' miRNA: 3'- gUCGaac-UCCGA---GUAG-GGUGCUGGUG- -5' |
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| 23259 | 3' | -55.7 | NC_005259.1 | + | 20795 | 0.67 | 0.64997 |
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Target: 5'- aCAGCaaGAGccaccuGCUCGUCCCgucGCGGCgCACc -3' miRNA: 3'- -GUCGaaCUC------CGAGUAGGG---UGCUG-GUG- -5' |
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| 23259 | 3' | -55.7 | NC_005259.1 | + | 46449 | 0.68 | 0.595383 |
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Target: 5'- aAGCUgcugcucggUGAGGCUCAgaUCCUugaGCGcugugGCCACg -3' miRNA: 3'- gUCGA---------ACUCCGAGU--AGGG---UGC-----UGGUG- -5' |
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| 23259 | 3' | -55.7 | NC_005259.1 | + | 35356 | 0.68 | 0.584522 |
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Target: 5'- gCAGCUUGuucaccaaguAGGCgguagGUCCgACGGCCAUg -3' miRNA: 3'- -GUCGAAC----------UCCGag---UAGGgUGCUGGUG- -5' |
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| 23259 | 3' | -55.7 | NC_005259.1 | + | 44339 | 0.69 | 0.520448 |
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Target: 5'- gUAGCUgccGGGCUUGUUCCACGGCUc- -3' miRNA: 3'- -GUCGAac-UCCGAGUAGGGUGCUGGug -5' |
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| 23259 | 3' | -55.7 | NC_005259.1 | + | 33953 | 1.09 | 0.001096 |
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Target: 5'- aCAGCUUGAGGCUCAUCCCACGACCACc -3' miRNA: 3'- -GUCGAACUCCGAGUAGGGUGCUGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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