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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23262 | 5' | -53.9 | NC_005259.1 | + | 58304 | 0.66 | 0.861663 |
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Target: 5'- cGGAUacCGCCACGGUuGAUGGugaUCUc -3' miRNA: 3'- aCCUA--GUGGUGCCAcCUGCCua-AGAc -5' |
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| 23262 | 5' | -53.9 | NC_005259.1 | + | 2020 | 0.68 | 0.749805 |
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Target: 5'- gUGGcgCACCggcaccucaguGCGGUGGGCGG--UCa- -3' miRNA: 3'- -ACCuaGUGG-----------UGCCACCUGCCuaAGac -5' |
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| 23262 | 5' | -53.9 | NC_005259.1 | + | 41616 | 0.69 | 0.664711 |
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Target: 5'- cGGAUCgcgaggcugACCAUGGUGG-CGGugccggUCUGc -3' miRNA: 3'- aCCUAG---------UGGUGCCACCuGCCua----AGAC- -5' |
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| 23262 | 5' | -53.9 | NC_005259.1 | + | 66593 | 0.71 | 0.577628 |
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Target: 5'- aUGGAUCGCCGCauccaugcaGGUGGggGCGGuauUUCUc -3' miRNA: 3'- -ACCUAGUGGUG---------CCACC--UGCCu--AAGAc -5' |
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| 23262 | 5' | -53.9 | NC_005259.1 | + | 33700 | 1.08 | 0.002213 |
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Target: 5'- aUGGAUCACCACGGUGGACGGAUUCUGc -3' miRNA: 3'- -ACCUAGUGGUGCCACCUGCCUAAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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