Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23269 | 5' | -49.4 | NC_005259.1 | + | 67432 | 0.66 | 0.964569 |
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Target: 5'- cCGGgcUCACGCUUGagcgcGUCGGGGAu -3' miRNA: 3'- -GCU--AGUGCGAAUgguaaCAGCCCUUu -5' |
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| 23269 | 5' | -49.4 | NC_005259.1 | + | 59861 | 0.67 | 0.956714 |
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Target: 5'- gGAUCAUGCUgGCuCGUUGUggccgaaacCGGGGGc -3' miRNA: 3'- gCUAGUGCGAaUG-GUAACA---------GCCCUUu -5' |
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| 23269 | 5' | -49.4 | NC_005259.1 | + | 44159 | 0.67 | 0.952373 |
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Target: 5'- gCGGUCGagcaGCgcGCCggUGUCGGaGAAAa -3' miRNA: 3'- -GCUAGUg---CGaaUGGuaACAGCC-CUUU- -5' |
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| 23269 | 5' | -49.4 | NC_005259.1 | + | 15783 | 0.67 | 0.952373 |
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Target: 5'- cCGAUCAUGCUgGCCGc---CGGGAu- -3' miRNA: 3'- -GCUAGUGCGAaUGGUaacaGCCCUuu -5' |
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| 23269 | 5' | -49.4 | NC_005259.1 | + | 42019 | 0.69 | 0.900242 |
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Target: 5'- gCGAUCACGCacgAggGUUGUCGGGu-- -3' miRNA: 3'- -GCUAGUGCGaa-UggUAACAGCCCuuu -5' |
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| 23269 | 5' | -49.4 | NC_005259.1 | + | 46517 | 0.7 | 0.834703 |
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Target: 5'- aCGAUCcCGCgaGCCg--GUCGGGGAu -3' miRNA: 3'- -GCUAGuGCGaaUGGuaaCAGCCCUUu -5' |
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| 23269 | 5' | -49.4 | NC_005259.1 | + | 29716 | 1.07 | 0.006876 |
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Target: 5'- cCGAUCACGCUUACCAUUGUCGGGAAAc -3' miRNA: 3'- -GCUAGUGCGAAUGGUAACAGCCCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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