miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2327 5' -55.3 NC_001416.1 + 30314 0.66 0.673568
Target:  5'- gCAUUGCCUgcuCUGCCGCUUcaCGCAGu -3'
miRNA:   3'- aGUAAUGGGaacGGUGGCGGA--GCGUC- -5'
2327 5' -55.3 NC_001416.1 + 20223 0.68 0.529477
Target:  5'- uUCAgugGCCUUUGCUgauGCCGCUUCuGCGc -3'
miRNA:   3'- -AGUaa-UGGGAACGG---UGGCGGAG-CGUc -5'
2327 5' -55.3 NC_001416.1 + 13076 0.67 0.561076
Target:  5'- ----cACCCUUGCgggccaugucauaCACCGCCUUcagaGCAGc -3'
miRNA:   3'- aguaaUGGGAACG-------------GUGGCGGAG----CGUC- -5'
2327 5' -55.3 NC_001416.1 + 11921 0.67 0.573201
Target:  5'- uUCAUaAUCCUUuuucGCCGCCGCCaUCaGCGu -3'
miRNA:   3'- -AGUAaUGGGAA----CGGUGGCGG-AG-CGUc -5'
2327 5' -55.3 NC_001416.1 + 6435 0.66 0.673568
Target:  5'- ----gACCCggcuuaCCGCCGCCgUCGCAu -3'
miRNA:   3'- aguaaUGGGaac---GGUGGCGG-AGCGUc -5'
2327 5' -55.3 NC_001416.1 + 5354 0.68 0.518729
Target:  5'- aUCAacaACUgUUucaGCCACUGCUUCGCAGg -3'
miRNA:   3'- -AGUaa-UGGgAA---CGGUGGCGGAGCGUC- -5'
2327 5' -55.3 NC_001416.1 + 352 1.09 0.000712
Target:  5'- cUCAUUACCCUUGCCACCGCCUCGCAGa -3'
miRNA:   3'- -AGUAAUGGGAACGGUGGCGGAGCGUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.