miRNA display CGI


Results 1 - 12 of 12 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23270 3' -50.9 NC_005259.1 + 19680 0.66 0.942525
Target:  5'- -cCCCGGUCagGUCgggaucgAGCAGGCUcgcacguUGGCu -3'
miRNA:   3'- gaGGGCCAGagUAG-------UCGUUUGA-------ACUG- -5'
23270 3' -50.9 NC_005259.1 + 61912 0.66 0.927337
Target:  5'- gUCgCCGGUCgUCGUCGGC-AGCUcGGu -3'
miRNA:   3'- gAG-GGCCAG-AGUAGUCGuUUGAaCUg -5'
23270 3' -50.9 NC_005259.1 + 2292 0.67 0.914461
Target:  5'- aUCCCGGUUUCugauacaGUCGGCAuuCUgguacagcGACu -3'
miRNA:   3'- gAGGGCCAGAG-------UAGUCGUuuGAa-------CUG- -5'
23270 3' -50.9 NC_005259.1 + 3294 0.67 0.894606
Target:  5'- cCUCgCCGGUC-CcUCGGCGAGCgcugcgucGACg -3'
miRNA:   3'- -GAG-GGCCAGaGuAGUCGUUUGaa------CUG- -5'
23270 3' -50.9 NC_005259.1 + 54484 0.68 0.879549
Target:  5'- aUCCCGccgaGggUCAUCGGCGGGCaUGGCg -3'
miRNA:   3'- gAGGGC----CagAGUAGUCGUUUGaACUG- -5'
23270 3' -50.9 NC_005259.1 + 58907 0.68 0.871617
Target:  5'- -cCUCGGgCUCGUCGGCGGuCUUGuCg -3'
miRNA:   3'- gaGGGCCaGAGUAGUCGUUuGAACuG- -5'
23270 3' -50.9 NC_005259.1 + 58971 0.68 0.854984
Target:  5'- --gUCGGgcagCUCGUCGGCGAcggcgaGCUUGGCc -3'
miRNA:   3'- gagGGCCa---GAGUAGUCGUU------UGAACUG- -5'
23270 3' -50.9 NC_005259.1 + 53958 0.69 0.818866
Target:  5'- -aCCCGGUgaacaUgGUCAGCAGGCcgguaUUGACg -3'
miRNA:   3'- gaGGGCCAg----AgUAGUCGUUUG-----AACUG- -5'
23270 3' -50.9 NC_005259.1 + 52970 0.69 0.818866
Target:  5'- -gCCCGG-CUgCAUCGGCAAGCaUGuGCa -3'
miRNA:   3'- gaGGGCCaGA-GUAGUCGUUUGaAC-UG- -5'
23270 3' -50.9 NC_005259.1 + 60212 0.7 0.789591
Target:  5'- uCUCgCCGaUCUCGUCGGCGcugAGCUcgcUGGCa -3'
miRNA:   3'- -GAG-GGCcAGAGUAGUCGU---UUGA---ACUG- -5'
23270 3' -50.9 NC_005259.1 + 49033 0.7 0.758773
Target:  5'- -cCCCGGUCgaGUCGGCAcgGACgaUGACg -3'
miRNA:   3'- gaGGGCCAGagUAGUCGU--UUGa-ACUG- -5'
23270 3' -50.9 NC_005259.1 + 29530 1.11 0.002375
Target:  5'- cCUCCCGGUCUCAUCAGCAAACUUGACg -3'
miRNA:   3'- -GAGGGCCAGAGUAGUCGUUUGAACUG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.