Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23271 | 3' | -55.1 | NC_005259.1 | + | 29420 | 1.1 | 0.0013 |
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Target: 5'- uCGUGCGCGCACACAGGAUGUACCUCGg -3' miRNA: 3'- -GCACGCGCGUGUGUCCUACAUGGAGC- -5' |
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| 23271 | 3' | -55.1 | NC_005259.1 | + | 28407 | 0.85 | 0.06483 |
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Target: 5'- gGUGCccGCGCGCAUGGGcAUGUACCUCGg -3' miRNA: 3'- gCACG--CGCGUGUGUCC-UACAUGGAGC- -5' |
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| 23271 | 3' | -55.1 | NC_005259.1 | + | 65049 | 0.82 | 0.116903 |
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Target: 5'- -cUGCGCGC-CACAGGuAUGUGCCUUGu -3' miRNA: 3'- gcACGCGCGuGUGUCC-UACAUGGAGC- -5' |
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| 23271 | 3' | -55.1 | NC_005259.1 | + | 3194 | 0.77 | 0.228471 |
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Target: 5'- gGUGCGCGCAC-CAcGAUGUgggGCCUCa -3' miRNA: 3'- gCACGCGCGUGuGUcCUACA---UGGAGc -5' |
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| 23271 | 3' | -55.1 | NC_005259.1 | + | 24261 | 0.75 | 0.316282 |
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Target: 5'- aGUGCGUGCACGCGuGGcUGgaagcgcucgACCUCGg -3' miRNA: 3'- gCACGCGCGUGUGU-CCuACa---------UGGAGC- -5' |
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| 23271 | 3' | -55.1 | NC_005259.1 | + | 41682 | 0.68 | 0.676673 |
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Target: 5'- gCGUGCguggccaccGCGCACACAGGcAUGcagccgccgcaUACCggCGg -3' miRNA: 3'- -GCACG---------CGCGUGUGUCC-UAC-----------AUGGa-GC- -5' |
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| 23271 | 3' | -55.1 | NC_005259.1 | + | 18861 | 0.66 | 0.789003 |
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Target: 5'- cCGUGCaacgguuGCGCGCGUGGGAggccaaccacgaUGUcgACCUCGg -3' miRNA: 3'- -GCACG-------CGCGUGUGUCCU------------ACA--UGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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