Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23275 | 5' | -56.7 | NC_005259.1 | + | 28311 | 1.08 | 0.000989 |
Target: 5'- cUGCGCAGCGUAGAUCGGUGUGCCGUCc -3' miRNA: 3'- -ACGCGUCGCAUCUAGCCACACGGCAG- -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 47173 | 0.8 | 0.097496 |
Target: 5'- cGUaGCGGCGaAGGUCGGcgGUGCCGUCg -3' miRNA: 3'- aCG-CGUCGCaUCUAGCCa-CACGGCAG- -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 33724 | 0.73 | 0.296919 |
Target: 5'- cUGCGCGaugaugucGCGUGGAgCGGUGUGCaCGa- -3' miRNA: 3'- -ACGCGU--------CGCAUCUaGCCACACG-GCag -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 56198 | 0.71 | 0.376603 |
Target: 5'- uUGCGCgAGCuGUgcucaaGGAUCGGUGUauGCCaGUCg -3' miRNA: 3'- -ACGCG-UCG-CA------UCUAGCCACA--CGG-CAG- -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 58332 | 0.71 | 0.376603 |
Target: 5'- cGgGCAGCGagaucAGGUCGGUGgccucggGCaCGUCg -3' miRNA: 3'- aCgCGUCGCa----UCUAGCCACa------CG-GCAG- -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 14090 | 0.7 | 0.440359 |
Target: 5'- gUGCGCAGCG-AGGUCGaGgGUGCgGa- -3' miRNA: 3'- -ACGCGUCGCaUCUAGC-CaCACGgCag -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 60581 | 0.69 | 0.520126 |
Target: 5'- gGCGgcccaCGGCG-AG-UCGG-GUGCCGUCa -3' miRNA: 3'- aCGC-----GUCGCaUCuAGCCaCACGGCAG- -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 64028 | 0.68 | 0.545185 |
Target: 5'- gGCGCGGCG-AGGcCGGUGaccggaucuuucucuUGCCGg- -3' miRNA: 3'- aCGCGUCGCaUCUaGCCAC---------------ACGGCag -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 11326 | 0.68 | 0.562106 |
Target: 5'- cGCGCAGCucgucGUCGGUcaUGCCGUUg -3' miRNA: 3'- aCGCGUCGcauc-UAGCCAc-ACGGCAG- -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 29954 | 0.68 | 0.572755 |
Target: 5'- gGuCGCGGCugGUGGggCGGgcgGUGaCCGUCc -3' miRNA: 3'- aC-GCGUCG--CAUCuaGCCa--CAC-GGCAG- -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 28750 | 0.68 | 0.58345 |
Target: 5'- cGCGCccgAGCGUgAGAUCGucgccgGUGCCGa- -3' miRNA: 3'- aCGCG---UCGCA-UCUAGCca----CACGGCag -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 8953 | 0.67 | 0.594184 |
Target: 5'- gGCGCGcCGUGGA-CGGgcaccGUGCCGg- -3' miRNA: 3'- aCGCGUcGCAUCUaGCCa----CACGGCag -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 54067 | 0.66 | 0.658934 |
Target: 5'- aGCGUuguccuGCGUgAGGUCGGUG-G-CGUCg -3' miRNA: 3'- aCGCGu-----CGCA-UCUAGCCACaCgGCAG- -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 38232 | 0.66 | 0.669698 |
Target: 5'- --aGUAGCGUGGAUCaGcgaugGUGCCGcCu -3' miRNA: 3'- acgCGUCGCAUCUAGcCa----CACGGCaG- -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 61495 | 0.66 | 0.691113 |
Target: 5'- cGCGaccguugaGGCGc---UCGGUGUGCCGgUCg -3' miRNA: 3'- aCGCg-------UCGCaucuAGCCACACGGC-AG- -5' |
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23275 | 5' | -56.7 | NC_005259.1 | + | 31511 | 0.66 | 0.691113 |
Target: 5'- cGCGCcggacuuGCuu-GAUCGGggugGUGCCGUg -3' miRNA: 3'- aCGCGu------CGcauCUAGCCa---CACGGCAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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