Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23280 | 5' | -58.1 | NC_005259.1 | + | 63864 | 0.66 | 0.621176 |
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Target: 5'- gAGGugGCCggcuGGCCUUGCg--UGGGc-- -3' miRNA: 3'- -UCCugUGG----CCGGAGCGacaACCCaug -5' |
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| 23280 | 5' | -58.1 | NC_005259.1 | + | 66948 | 0.66 | 0.620107 |
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Target: 5'- cGGGACACCGGCga-GCUGUUucuugcgcgccucGcGGUGa -3' miRNA: 3'- -UCCUGUGGCCGgagCGACAA-------------C-CCAUg -5' |
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| 23280 | 5' | -58.1 | NC_005259.1 | + | 25507 | 0.66 | 0.609418 |
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Target: 5'- gAGGGCAaaaaGGCCcucgaccguggcaUCGCg--UGGGUGCu -3' miRNA: 3'- -UCCUGUgg--CCGG-------------AGCGacaACCCAUG- -5' |
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| 23280 | 5' | -58.1 | NC_005259.1 | + | 271 | 0.66 | 0.595553 |
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Target: 5'- uAGGGCgguguacgccgccuACgCGGCCUCGCgcugaucucgaccggGGGUGCg -3' miRNA: 3'- -UCCUG--------------UG-GCCGGAGCGacaa-----------CCCAUG- -5' |
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| 23280 | 5' | -58.1 | NC_005259.1 | + | 50453 | 0.67 | 0.557467 |
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Target: 5'- gGGGuCGCCGGUCUCGCUGa------- -3' miRNA: 3'- -UCCuGUGGCCGGAGCGACaacccaug -5' |
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| 23280 | 5' | -58.1 | NC_005259.1 | + | 36918 | 0.68 | 0.475858 |
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Target: 5'- gGGGAUAUCGuGCCgacggugagugUUGCUGUcaccgGGGUGCc -3' miRNA: 3'- -UCCUGUGGC-CGG-----------AGCGACAa----CCCAUG- -5' |
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| 23280 | 5' | -58.1 | NC_005259.1 | + | 25706 | 1.08 | 0.000758 |
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Target: 5'- aAGGACACCGGCCUCGCUGUUGGGUACc -3' miRNA: 3'- -UCCUGUGGCCGGAGCGACAACCCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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