Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23281 | 3' | -54.7 | NC_005259.1 | + | 37553 | 0.66 | 0.799531 |
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Target: 5'- -gCGUGUGGUguccgaugUCGACugcucgCcgCCCGCg -3' miRNA: 3'- aaGCACGCCA--------AGCUGua----GuaGGGCGa -5' |
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| 23281 | 3' | -54.7 | NC_005259.1 | + | 61434 | 0.66 | 0.779997 |
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Target: 5'- ---uUGCGGgcgcgccggUCGAUcUCAUCCUGCUg -3' miRNA: 3'- aagcACGCCa--------AGCUGuAGUAGGGCGA- -5' |
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| 23281 | 3' | -54.7 | NC_005259.1 | + | 41107 | 0.66 | 0.779997 |
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Target: 5'- gUCG-GCGGUaugaCGAUGUCGgcggugCCCGCg -3' miRNA: 3'- aAGCaCGCCAa---GCUGUAGUa-----GGGCGa -5' |
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| 23281 | 3' | -54.7 | NC_005259.1 | + | 40893 | 0.66 | 0.759855 |
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Target: 5'- -cCGUgGCGGggUCGGCAgCGUCCCacGCUu -3' miRNA: 3'- aaGCA-CGCCa-AGCUGUaGUAGGG--CGA- -5' |
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| 23281 | 3' | -54.7 | NC_005259.1 | + | 34596 | 0.7 | 0.544358 |
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Target: 5'- -aCGcGCGGUgUCGACAUCAcucgUCCGCg -3' miRNA: 3'- aaGCaCGCCA-AGCUGUAGUa---GGGCGa -5' |
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| 23281 | 3' | -54.7 | NC_005259.1 | + | 25317 | 1.05 | 0.002729 |
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Target: 5'- gUUCGUGCGGUUCGACAUCAUCCCGCUc -3' miRNA: 3'- -AAGCACGCCAAGCUGUAGUAGGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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