Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23284 | 5' | -59.5 | NC_005259.1 | + | 65279 | 0.66 | 0.556728 |
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Target: 5'- uGUGUGGGuGcUGCCGuGGaucuucggcaaGgUCAUCGAg -3' miRNA: 3'- -CACACCC-C-ACGGCuCCg----------CgAGUAGCU- -5' |
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| 23284 | 5' | -59.5 | NC_005259.1 | + | 58674 | 0.66 | 0.536102 |
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Target: 5'- ---cGGGGUGCagcuuccaucgCGGGGUcgGCUCGUCGc -3' miRNA: 3'- cacaCCCCACG-----------GCUCCG--CGAGUAGCu -5' |
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| 23284 | 5' | -59.5 | NC_005259.1 | + | 67425 | 0.66 | 0.536102 |
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Target: 5'- ---cGGGGUGCCGGGcucaCGCUugagcgCGUCGGg -3' miRNA: 3'- cacaCCCCACGGCUCc---GCGA------GUAGCU- -5' |
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| 23284 | 5' | -59.5 | NC_005259.1 | + | 55035 | 0.68 | 0.401594 |
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Target: 5'- ---cGGGcUGCCGAGGUGCUUGccUCGGu -3' miRNA: 3'- cacaCCCcACGGCUCCGCGAGU--AGCU- -5' |
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| 23284 | 5' | -59.5 | NC_005259.1 | + | 26828 | 0.71 | 0.290559 |
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Target: 5'- ---cGGuGGUGCCGGGGCGgUCugcUCGGg -3' miRNA: 3'- cacaCC-CCACGGCUCCGCgAGu--AGCU- -5' |
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| 23284 | 5' | -59.5 | NC_005259.1 | + | 27997 | 0.71 | 0.276813 |
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Target: 5'- -gGUGcGGGUGCCGGGGCGgggUCGcCGGg -3' miRNA: 3'- caCAC-CCCACGGCUCCGCg--AGUaGCU- -5' |
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| 23284 | 5' | -59.5 | NC_005259.1 | + | 41636 | 0.75 | 0.158126 |
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Target: 5'- -gGUGGcGGUGCCGgucugcGGGCGCUCggUGAg -3' miRNA: 3'- caCACC-CCACGGC------UCCGCGAGuaGCU- -5' |
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| 23284 | 5' | -59.5 | NC_005259.1 | + | 24745 | 1.08 | 0.000579 |
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Target: 5'- gGUGUGGGGUGCCGAGGCGCUCAUCGAa -3' miRNA: 3'- -CACACCCCACGGCUCCGCGAGUAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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