miRNA: 3'- cauaCGGGU--UCGaGuaGCCGUAGGGc -5'
miRNA: 3'- cauaCGGGU-UCGAGUaGCCGuagGGC- -5'
miRNA: 3'- cauacGGGUUCGAGUAGCCG-UAGggC- -5'
miRNA: 3'- -CAUACGGGU-UCGaGUAGCCgUa--G-GGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23285 | 3' | -54.9 | NC_005259.1 | + | 35113 | 0.66 | 0.758122 |
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Target: 5'- ---cGCCCGuuGGCcCggCGGUGUCCCa -3' miRNA: 3'- cauaCGGGU--UCGaGuaGCCGUAGGGc -5' |
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| 23285 | 3' | -54.9 | NC_005259.1 | + | 40873 | 0.66 | 0.758122 |
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Target: 5'- ---cGCCCAcAGCUCAcCGGCca-CCGu -3' miRNA: 3'- cauaCGGGU-UCGAGUaGCCGuagGGC- -5' |
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| 23285 | 3' | -54.9 | NC_005259.1 | + | 58907 | 0.66 | 0.758122 |
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Target: 5'- ----cCUCGGGCUCGUCGGCgGUCuuGu -3' miRNA: 3'- cauacGGGUUCGAGUAGCCG-UAGggC- -5' |
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| 23285 | 3' | -54.9 | NC_005259.1 | + | 19553 | 0.66 | 0.768293 |
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Target: 5'- cGUAUGCCCGcAGC-CGUCGGgAcagCgCCGc -3' miRNA: 3'- -CAUACGGGU-UCGaGUAGCCgUa--G-GGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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