Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23289 | 5' | -53.6 | NC_005259.1 | + | 20182 | 0.66 | 0.861419 |
Target: 5'- cGCCUCGGCuGACGaGAUCGccGGuugcAAGa -3' miRNA: 3'- cCGGAGCCG-CUGCaCUAGC--UCu---UUCa -5' |
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23289 | 5' | -53.6 | NC_005259.1 | + | 67909 | 0.66 | 0.861419 |
Target: 5'- gGGUCUCGGCGAgGUcaagcgcguaGGUCGcGAGGa- -3' miRNA: 3'- -CCGGAGCCGCUgCA----------CUAGCuCUUUca -5' |
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23289 | 5' | -53.6 | NC_005259.1 | + | 17599 | 0.66 | 0.853212 |
Target: 5'- -aCCUCGGCgcgauGACGUGGggcggCGAGGAcGGUu -3' miRNA: 3'- ccGGAGCCG-----CUGCACUa----GCUCUU-UCA- -5' |
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23289 | 5' | -53.6 | NC_005259.1 | + | 55052 | 0.67 | 0.836129 |
Target: 5'- uGCCUCGGUGGCagucUGcgCGAGAu--- -3' miRNA: 3'- cCGGAGCCGCUGc---ACuaGCUCUuuca -5' |
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23289 | 5' | -53.6 | NC_005259.1 | + | 36159 | 0.67 | 0.82727 |
Target: 5'- uGGCCaCGGUGACGaccuugccgaGGUCGAGAGc-- -3' miRNA: 3'- -CCGGaGCCGCUGCa---------CUAGCUCUUuca -5' |
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23289 | 5' | -53.6 | NC_005259.1 | + | 33319 | 0.67 | 0.82727 |
Target: 5'- cGCCgUCGGCGGucacgcggaaGUGAUCGGGGucGa -3' miRNA: 3'- cCGG-AGCCGCUg---------CACUAGCUCUuuCa -5' |
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23289 | 5' | -53.6 | NC_005259.1 | + | 4999 | 0.68 | 0.780166 |
Target: 5'- gGGCaCUaCGGCaAgGUGGUCGAGAucGa -3' miRNA: 3'- -CCG-GA-GCCGcUgCACUAGCUCUuuCa -5' |
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23289 | 5' | -53.6 | NC_005259.1 | + | 64811 | 0.68 | 0.770254 |
Target: 5'- cGGCCUCGGCGGCaaUGAgCGuGu-GGUg -3' miRNA: 3'- -CCGGAGCCGCUGc-ACUaGCuCuuUCA- -5' |
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23289 | 5' | -53.6 | NC_005259.1 | + | 8449 | 0.68 | 0.750025 |
Target: 5'- uGGUCUCGGU---GUGGUCGAGguAGa -3' miRNA: 3'- -CCGGAGCCGcugCACUAGCUCuuUCa -5' |
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23289 | 5' | -53.6 | NC_005259.1 | + | 54505 | 0.69 | 0.708253 |
Target: 5'- gGGCauggCGGCGAUGUGGgguggCGGGucGAGUa -3' miRNA: 3'- -CCGga--GCCGCUGCACUa----GCUCu-UUCA- -5' |
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23289 | 5' | -53.6 | NC_005259.1 | + | 5553 | 0.69 | 0.686887 |
Target: 5'- aGGCCgcgUGGgGugGUGAcggCGAGAccGUg -3' miRNA: 3'- -CCGGa--GCCgCugCACUa--GCUCUuuCA- -5' |
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23289 | 5' | -53.6 | NC_005259.1 | + | 29406 | 0.72 | 0.557178 |
Target: 5'- gGGCCUCGGCGAgcuCGUGcgCGcacacAGGAuGUa -3' miRNA: 3'- -CCGGAGCCGCU---GCACuaGC-----UCUUuCA- -5' |
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23289 | 5' | -53.6 | NC_005259.1 | + | 34024 | 0.8 | 0.204416 |
Target: 5'- cGGCCUCGGCGGCGUGcGUCGGc----- -3' miRNA: 3'- -CCGGAGCCGCUGCAC-UAGCUcuuuca -5' |
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23289 | 5' | -53.6 | NC_005259.1 | + | 22393 | 1.1 | 0.002023 |
Target: 5'- cGGCCUCGGCGACGUGAUCGAGAAAGUc -3' miRNA: 3'- -CCGGAGCCGCUGCACUAGCUCUUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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