Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23290 | 5' | -55.5 | NC_005259.1 | + | 36147 | 0.66 | 0.76532 |
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Target: 5'- uGUCCGUCGccaUGGccacggUGaCGACCUUGCc- -3' miRNA: 3'- gCGGGUAGC---ACCa-----AC-GCUGGAACGuc -5' |
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| 23290 | 5' | -55.5 | NC_005259.1 | + | 57126 | 0.66 | 0.755227 |
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Target: 5'- uCGCCCAUCGgc---GgGACCgaGCAGu -3' miRNA: 3'- -GCGGGUAGCaccaaCgCUGGaaCGUC- -5' |
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| 23290 | 5' | -55.5 | NC_005259.1 | + | 17373 | 0.67 | 0.671011 |
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Target: 5'- gCGCUgGUCGUGGUcgacUGCGACacUGCc- -3' miRNA: 3'- -GCGGgUAGCACCA----ACGCUGgaACGuc -5' |
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| 23290 | 5' | -55.5 | NC_005259.1 | + | 67155 | 0.68 | 0.660213 |
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Target: 5'- cCGCUUGUCGaUGGUgGCGGCCaUGCc- -3' miRNA: 3'- -GCGGGUAGC-ACCAaCGCUGGaACGuc -5' |
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| 23290 | 5' | -55.5 | NC_005259.1 | + | 58544 | 0.68 | 0.627705 |
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Target: 5'- uGCCCGUCGaGGUUaCGGCUcggGCGGu -3' miRNA: 3'- gCGGGUAGCaCCAAcGCUGGaa-CGUC- -5' |
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| 23290 | 5' | -55.5 | NC_005259.1 | + | 48261 | 0.71 | 0.470039 |
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Target: 5'- uGCUguaGUCGUacuuacGGUUGuCGACCUUGCGGu -3' miRNA: 3'- gCGGg--UAGCA------CCAAC-GCUGGAACGUC- -5' |
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| 23290 | 5' | -55.5 | NC_005259.1 | + | 17466 | 0.75 | 0.289568 |
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Target: 5'- cCGCCCAcCGUGGUgacgccCGGCCaUGCGGg -3' miRNA: 3'- -GCGGGUaGCACCAac----GCUGGaACGUC- -5' |
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| 23290 | 5' | -55.5 | NC_005259.1 | + | 22181 | 1.09 | 0.00119 |
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Target: 5'- gCGCCCAUCGUGGUUGCGACCUUGCAGa -3' miRNA: 3'- -GCGGGUAGCACCAACGCUGGAACGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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