Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23291 | 3' | -60.6 | NC_005259.1 | + | 35260 | 0.66 | 0.468176 |
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Target: 5'- cGUUGUUCgCgCCCGCC-GCGCCGGgGu -3' miRNA: 3'- -CAGCAAGaG-GGGUGGcCGUGGCCgUu -5' |
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| 23291 | 3' | -60.6 | NC_005259.1 | + | 22689 | 0.66 | 0.468176 |
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Target: 5'- -cCGaUUUCCCCGCCGucgccguCACCGGCu- -3' miRNA: 3'- caGCaAGAGGGGUGGCc------GUGGCCGuu -5' |
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| 23291 | 3' | -60.6 | NC_005259.1 | + | 61544 | 0.67 | 0.421267 |
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Target: 5'- cUCG-UCUCgggcgagaaugCCgACaCGGCACCGGCAGu -3' miRNA: 3'- cAGCaAGAG-----------GGgUG-GCCGUGGCCGUU- -5' |
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| 23291 | 3' | -60.6 | NC_005259.1 | + | 34157 | 0.67 | 0.394518 |
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Target: 5'- gGUCGUggugCCCCACCaGUGCCGGa-- -3' miRNA: 3'- -CAGCAaga-GGGGUGGcCGUGGCCguu -5' |
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| 23291 | 3' | -60.6 | NC_005259.1 | + | 34354 | 0.67 | 0.385849 |
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Target: 5'- -aCGUgacCCaCCGCCGGUAUCGGCGc -3' miRNA: 3'- caGCAagaGG-GGUGGCCGUGGCCGUu -5' |
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| 23291 | 3' | -60.6 | NC_005259.1 | + | 51351 | 0.68 | 0.368892 |
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Target: 5'- cUCGgauaUgCCCGgUGGCACCGGCAc -3' miRNA: 3'- cAGCaag-AgGGGUgGCCGUGGCCGUu -5' |
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| 23291 | 3' | -60.6 | NC_005259.1 | + | 57119 | 0.69 | 0.299082 |
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Target: 5'- aUCGUcCUCgCCCAUCGGCgggACCGaGCAGu -3' miRNA: 3'- cAGCAaGAG-GGGUGGCCG---UGGC-CGUU- -5' |
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| 23291 | 3' | -60.6 | NC_005259.1 | + | 30990 | 0.7 | 0.271501 |
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Target: 5'- cUCGUUgUCCCCGCCguGGgGCCGGa-- -3' miRNA: 3'- cAGCAAgAGGGGUGG--CCgUGGCCguu -5' |
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| 23291 | 3' | -60.6 | NC_005259.1 | + | 58734 | 0.71 | 0.239945 |
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Target: 5'- uGUCGUUgg---CACCGGCACCGGCGAa -3' miRNA: 3'- -CAGCAAgagggGUGGCCGUGGCCGUU- -5' |
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| 23291 | 3' | -60.6 | NC_005259.1 | + | 21979 | 1.05 | 0.000675 |
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Target: 5'- uGUCGUUCUCCCCACCGGCACCGGCAAg -3' miRNA: 3'- -CAGCAAGAGGGGUGGCCGUGGCCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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