Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23295 | 3' | -55.8 | NC_005259.1 | + | 51082 | 0.66 | 0.73823 |
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Target: 5'- gGUGGcaugGCCCacg-AUAACGAGGGCg -3' miRNA: 3'- aCACCca--CGGGcagaUGUUGCUCCUG- -5' |
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| 23295 | 3' | -55.8 | NC_005259.1 | + | 41636 | 0.66 | 0.717381 |
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Target: 5'- gGUGgcGGUGCCgGUCUGCGggcgcucggugaGCG-GGAUc -3' miRNA: 3'- aCAC--CCACGGgCAGAUGU------------UGCuCCUG- -5' |
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| 23295 | 3' | -55.8 | NC_005259.1 | + | 3209 | 0.67 | 0.696205 |
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Target: 5'- aUGUGGG-GCCUcaagcacuuuGUgCUGCGcuACGAGGAa -3' miRNA: 3'- -ACACCCaCGGG----------CA-GAUGU--UGCUCCUg -5' |
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| 23295 | 3' | -55.8 | NC_005259.1 | + | 7683 | 0.67 | 0.66078 |
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Target: 5'- cGUGGGgugGCCCGuacgUCUGCAccaccaucgucuggGcCGAGGGa -3' miRNA: 3'- aCACCCa--CGGGC----AGAUGU--------------U-GCUCCUg -5' |
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| 23295 | 3' | -55.8 | NC_005259.1 | + | 63936 | 0.68 | 0.631569 |
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Target: 5'- cGUGGGUcuuaCCGUCcgGCAGCGAGaucucGGCg -3' miRNA: 3'- aCACCCAcg--GGCAGa-UGUUGCUC-----CUG- -5' |
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| 23295 | 3' | -55.8 | NC_005259.1 | + | 19977 | 1.1 | 0.000931 |
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Target: 5'- gUGUGGGUGCCCGUCUACAACGAGGACg -3' miRNA: 3'- -ACACCCACGGGCAGAUGUUGCUCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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