Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
233 | 5' | -56.9 | AC_000008.1 | + | 22231 | 0.66 | 0.539947 |
Target: 5'- uCUACagcuUCCuGGAG-CGCCACUCGCc -3' miRNA: 3'- -GGUGauu-GGGcCCUCgGUGGUGAGCG- -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 11726 | 0.66 | 0.536712 |
Target: 5'- gCGCUGcuaaacauaguagaGCCCGaGGGCCGCUggcuGCUCGa -3' miRNA: 3'- gGUGAU--------------UGGGCcCUCGGUGG----UGAGCg -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 23560 | 0.66 | 0.529193 |
Target: 5'- aCCACgcGCCacuGGGucgucuucauucAGCCGCCGCacugugCGCu -3' miRNA: 3'- -GGUGauUGGg--CCC------------UCGGUGGUGa-----GCG- -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 15980 | 0.66 | 0.518519 |
Target: 5'- aCCGCgcGCCCuGGGGCgCGCaCAaaCGCg -3' miRNA: 3'- -GGUGauUGGGcCCUCG-GUG-GUgaGCG- -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 16762 | 0.66 | 0.497436 |
Target: 5'- aCCACcGuagucuuuacGCCCGGuGAGCgCuCCACcCGCa -3' miRNA: 3'- -GGUGaU----------UGGGCC-CUCG-GuGGUGaGCG- -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 17796 | 0.67 | 0.48704 |
Target: 5'- uCCACagcgAGCCCcagcugaaGGcGCCACCGCUCa- -3' miRNA: 3'- -GGUGa---UUGGGc-------CCuCGGUGGUGAGcg -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 19540 | 0.67 | 0.475724 |
Target: 5'- gCACUAAUggCCGcGGcugcugcGGCgGCCGCUCGUc -3' miRNA: 3'- gGUGAUUG--GGC-CC-------UCGgUGGUGAGCG- -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 35309 | 0.68 | 0.436711 |
Target: 5'- aCCGaaaUAGCCCGGGGGaauaCAuacCCGCagGCg -3' miRNA: 3'- -GGUg--AUUGGGCCCUCg---GU---GGUGagCG- -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 3795 | 0.68 | 0.417432 |
Target: 5'- gCCGCUucAGCCgCuGcAGCCACCGCcCGCg -3' miRNA: 3'- -GGUGA--UUGG-GcCcUCGGUGGUGaGCG- -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 8776 | 0.68 | 0.407988 |
Target: 5'- -uGCUGGCgCCGGGuGUgGCCGCUgGa -3' miRNA: 3'- ggUGAUUG-GGCCCuCGgUGGUGAgCg -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 26466 | 0.68 | 0.393161 |
Target: 5'- aCCACUGgaACCaGGGccgguaaguccaagcAGCCGCCGC-CGUu -3' miRNA: 3'- -GGUGAU--UGGgCCC---------------UCGGUGGUGaGCG- -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 17502 | 0.69 | 0.371585 |
Target: 5'- aCCACUGgaACCCGcc-GCCGCCG-UCGCc -3' miRNA: 3'- -GGUGAU--UGGGCccuCGGUGGUgAGCG- -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 25528 | 0.69 | 0.344099 |
Target: 5'- aCGCUGGCCCcucuaccgccAGCCGCCGC-CGCa -3' miRNA: 3'- gGUGAUUGGGccc-------UCGGUGGUGaGCG- -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 16190 | 0.71 | 0.276233 |
Target: 5'- gCCGCcGACCCGGcacuGCCGCCcaACgCGCg -3' miRNA: 3'- -GGUGaUUGGGCCcu--CGGUGG--UGaGCG- -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 15707 | 0.74 | 0.175876 |
Target: 5'- cUCACaGAUCaCGGGAcGCUACCGCUgCGCa -3' miRNA: 3'- -GGUGaUUGG-GCCCU-CGGUGGUGA-GCG- -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 31774 | 0.76 | 0.122436 |
Target: 5'- aCCGCUGGuCCCGGGc-CUACCGCgcgCGCa -3' miRNA: 3'- -GGUGAUU-GGGCCCucGGUGGUGa--GCG- -5' |
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233 | 5' | -56.9 | AC_000008.1 | + | 15539 | 1.13 | 0.000244 |
Target: 5'- uCCACUAACCCGGGAGCCACCACUCGCu -3' miRNA: 3'- -GGUGAUUGGGCCCUCGGUGGUGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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