Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2330 | 5' | -54.6 | NC_001416.1 | + | 1191 | 0.66 | 0.716549 |
Target: 5'- aGCGUGuUAUCCCGGUGCUuuuugccauaccacgGgGCCaGCg -3' miRNA: 3'- cUGCAUuAUAGGGUCGCGG---------------CgUGG-CG- -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 40274 | 0.66 | 0.72304 |
Target: 5'- gGACGcaUAAUAgcuUCUguGCGCCGgacguUGCCGCg -3' miRNA: 3'- -CUGC--AUUAU---AGGguCGCGGC-----GUGGCG- -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 8231 | 0.66 | 0.72304 |
Target: 5'- cGCGgguUGUCCUuucGCGCCGCAggcgUCGUa -3' miRNA: 3'- cUGCauuAUAGGGu--CGCGGCGU----GGCG- -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 16613 | 0.66 | 0.701287 |
Target: 5'- uGugGUGAUG-CCgGaugauggcGCGCCGUuCCGCu -3' miRNA: 3'- -CugCAUUAUaGGgU--------CGCGGCGuGGCG- -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 15986 | 0.66 | 0.701287 |
Target: 5'- gGugGUGAUGgguaaCCuGcCGCCGCGcCCGUu -3' miRNA: 3'- -CugCAUUAUag---GGuC-GCGGCGU-GGCG- -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 7901 | 0.66 | 0.6903 |
Target: 5'- cGAUGgucagcGUGUCuCCA-CGCCGCagcuGCCGCa -3' miRNA: 3'- -CUGCau----UAUAG-GGUcGCGGCG----UGGCG- -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 16408 | 0.66 | 0.72304 |
Target: 5'- aGACGUu---UCCCcaUGCCGUAgCGCg -3' miRNA: 3'- -CUGCAuuauAGGGucGCGGCGUgGCG- -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 8024 | 0.67 | 0.642544 |
Target: 5'- cGGCGgug---CCUGGUGCCGCcgcaauggccauuaACCGCg -3' miRNA: 3'- -CUGCauuauaGGGUCGCGGCG--------------UGGCG- -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 17466 | 0.67 | 0.65704 |
Target: 5'- --gGU-GUGUCCCAucGCGCCaGCACCu- -3' miRNA: 3'- cugCAuUAUAGGGU--CGCGG-CGUGGcg -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 5504 | 0.67 | 0.65704 |
Target: 5'- cGCGUucacGUCCgGcuGCGCCGCGCUGg -3' miRNA: 3'- cUGCAuua-UAGGgU--CGCGGCGUGGCg -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 16327 | 0.68 | 0.590158 |
Target: 5'- aACGUuccGUCCCAGauacCGCUGUauuGCCGCg -3' miRNA: 3'- cUGCAuuaUAGGGUC----GCGGCG---UGGCG- -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 3777 | 0.7 | 0.451838 |
Target: 5'- cGCGUAuUAcgCCgCAGCGCCGguCCGg -3' miRNA: 3'- cUGCAUuAUa-GG-GUCGCGGCguGGCg -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 17600 | 0.71 | 0.41284 |
Target: 5'- -cUGUAGUucCCCAGCGCCaGUugCGUg -3' miRNA: 3'- cuGCAUUAuaGGGUCGCGG-CGugGCG- -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 17860 | 0.71 | 0.394136 |
Target: 5'- uGGCGgc-UAUCCaguaCAGCGCCGUACCa- -3' miRNA: 3'- -CUGCauuAUAGG----GUCGCGGCGUGGcg -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 17598 | 0.71 | 0.403419 |
Target: 5'- cGGCGUcGGUAUCguuCCGGauuGCCGCACCGg -3' miRNA: 3'- -CUGCA-UUAUAG---GGUCg--CGGCGUGGCg -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 5092 | 0.72 | 0.353256 |
Target: 5'- aGCGUGAUuuccacacccuguuUCuCCAGCGCaGCACCGUa -3' miRNA: 3'- cUGCAUUAu-------------AG-GGUCGCGgCGUGGCG- -5' |
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2330 | 5' | -54.6 | NC_001416.1 | + | 3499 | 1.11 | 0.000691 |
Target: 5'- uGACGUAAUAUCCCAGCGCCGCACCGCu -3' miRNA: 3'- -CUGCAUUAUAGGGUCGCGGCGUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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