Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23300 | 3' | -52.6 | NC_005259.1 | + | 54083 | 0.66 | 0.909741 |
Target: 5'- aGgUCGGUGGCgUCGcgCAUCaccggGGUCAa -3' miRNA: 3'- -CgAGCCGCUG-AGCuaGUAGga---CUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 19853 | 0.66 | 0.903123 |
Target: 5'- cGCUCGGUGuggcGCUCGcgCccgCCggGAUCGc -3' miRNA: 3'- -CGAGCCGC----UGAGCuaGua-GGa-CUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 53020 | 0.66 | 0.903123 |
Target: 5'- cGCUgCGGUGGCgUCGAggCAUCgUGAccUCGc -3' miRNA: 3'- -CGA-GCCGCUG-AGCUa-GUAGgACU--AGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 52912 | 0.66 | 0.896236 |
Target: 5'- uGCUCacgaccGcCGACUCGAUgG-CCUGGUCGc -3' miRNA: 3'- -CGAGc-----C-GCUGAGCUAgUaGGACUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 47325 | 0.66 | 0.896236 |
Target: 5'- --cCGGCGGcCUUGGUCAgccCCgUGAUCGu -3' miRNA: 3'- cgaGCCGCU-GAGCUAGUa--GG-ACUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 61509 | 0.66 | 0.88167 |
Target: 5'- cGCUCGGUGuGC-CGGUCGaCCUcGUCGg -3' miRNA: 3'- -CGAGCCGC-UGaGCUAGUaGGAcUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 10430 | 0.66 | 0.88167 |
Target: 5'- cCUCGGCGAgCUCGAcaucgaguggcUCGacggcggugUCCUGcgCAg -3' miRNA: 3'- cGAGCCGCU-GAGCU-----------AGU---------AGGACuaGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 50448 | 0.67 | 0.857923 |
Target: 5'- gGCUCGGgGucGC-CGGUC-UCgCUGAUCAc -3' miRNA: 3'- -CGAGCCgC--UGaGCUAGuAG-GACUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 15939 | 0.67 | 0.857923 |
Target: 5'- cGCUCGGCGAaaugGAUgGUCaaGGUCGa -3' miRNA: 3'- -CGAGCCGCUgag-CUAgUAGgaCUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 51797 | 0.68 | 0.832063 |
Target: 5'- cGCUCGGCGGgUCGGUCAgCUcaggCAg -3' miRNA: 3'- -CGAGCCGCUgAGCUAGUaGGacuaGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 62866 | 0.69 | 0.788845 |
Target: 5'- cGCUCGGCGcgagguaggccGCUCGGUCAuugagcgacgccgcuUCCUcGGUa- -3' miRNA: 3'- -CGAGCCGC-----------UGAGCUAGU---------------AGGA-CUAgu -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 29230 | 0.69 | 0.78491 |
Target: 5'- cCUCGGCGcguuCcUGAUCAUCCUGuccAUCGu -3' miRNA: 3'- cGAGCCGCu---GaGCUAGUAGGAC---UAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 15219 | 0.69 | 0.774961 |
Target: 5'- cGCUCGGCG-CUCGG-CAUCaacGUCGa -3' miRNA: 3'- -CGAGCCGCuGAGCUaGUAGgacUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 66465 | 0.69 | 0.774961 |
Target: 5'- aCUCGGUGACgcugguggCGGUgGUCUcGAUCAu -3' miRNA: 3'- cGAGCCGCUGa-------GCUAgUAGGaCUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 13115 | 0.69 | 0.761811 |
Target: 5'- cGCUCGGCGGCUaCGAccagcgcacgguguUCggCUcGAUCAu -3' miRNA: 3'- -CGAGCCGCUGA-GCU--------------AGuaGGaCUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 42770 | 0.71 | 0.625384 |
Target: 5'- uGCUCGGCGGuCUCGGggCcgCCaccGAUCAc -3' miRNA: 3'- -CGAGCCGCU-GAGCUa-GuaGGa--CUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 10930 | 0.73 | 0.559915 |
Target: 5'- aGCUCGGCGaACUCGGugUCGaCCUcGUCGg -3' miRNA: 3'- -CGAGCCGC-UGAGCU--AGUaGGAcUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 23015 | 0.73 | 0.517306 |
Target: 5'- ---aGGaCGGCUCGGUCAUCgaGAUCGa -3' miRNA: 3'- cgagCC-GCUGAGCUAGUAGgaCUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 17359 | 0.74 | 0.486218 |
Target: 5'- aGgUCGGCGGCUCGGcgcUgGUCgUGGUCGa -3' miRNA: 3'- -CgAGCCGCUGAGCU---AgUAGgACUAGU- -5' |
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23300 | 3' | -52.6 | NC_005259.1 | + | 60106 | 0.76 | 0.354934 |
Target: 5'- aGCUCGaccaGCGACUCGAuaucggcggccUCGUCCUGcgCGg -3' miRNA: 3'- -CGAGC----CGCUGAGCU-----------AGUAGGACuaGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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