Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23301 | 3' | -56.6 | NC_005259.1 | + | 67963 | 0.66 | 0.67638 |
Target: 5'- -uGUGCUguagcgcgcgcagCGGCGUGGCCaccGCCGCGa- -3' miRNA: 3'- acCACGA-------------GCUGUACCGGa--CGGUGUag -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 9578 | 0.66 | 0.65575 |
Target: 5'- aGGUGCUCGuCAacgucgucggucUGGCCguagagcaccGUgGCAUCg -3' miRNA: 3'- aCCACGAGCuGU------------ACCGGa---------CGgUGUAG- -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 42769 | 0.66 | 0.644857 |
Target: 5'- --cUGCUCGGCGgucucggGGCC-GCCACcgAUCa -3' miRNA: 3'- accACGAGCUGUa------CCGGaCGGUG--UAG- -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 17738 | 0.66 | 0.644857 |
Target: 5'- gUGGcUGCUCGACGcgauccgugaGGCC-GCCGCGc- -3' miRNA: 3'- -ACC-ACGAGCUGUa---------CCGGaCGGUGUag -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 57453 | 0.66 | 0.633952 |
Target: 5'- uUGGUGCgacgcugCGGCA-GGCCggugGCaccggaCACGUCg -3' miRNA: 3'- -ACCACGa------GCUGUaCCGGa---CG------GUGUAG- -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 47955 | 0.67 | 0.623046 |
Target: 5'- cGGUGC-CGA--UGGCC-GCCGCGc- -3' miRNA: 3'- aCCACGaGCUguACCGGaCGGUGUag -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 12553 | 0.67 | 0.612147 |
Target: 5'- cGGcauUGC-CGGgGUGGCCcGCCAgAUCc -3' miRNA: 3'- aCC---ACGaGCUgUACCGGaCGGUgUAG- -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 60566 | 0.67 | 0.60888 |
Target: 5'- cGGUGC-CGACGgucaggcGGCCcacggcgagucgggUGCCGuCAUCg -3' miRNA: 3'- aCCACGaGCUGUa------CCGG--------------ACGGU-GUAG- -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 24299 | 0.67 | 0.583913 |
Target: 5'- aUGGUGCUgcugcgcgaucuaccCGACAUGGUCcgGCCuCAc- -3' miRNA: 3'- -ACCACGA---------------GCUGUACCGGa-CGGuGUag -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 46231 | 0.67 | 0.579589 |
Target: 5'- uUGGUcaGCUCGGCugccGCCUGaggCACGUCg -3' miRNA: 3'- -ACCA--CGAGCUGuac-CGGACg--GUGUAG- -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 1434 | 0.67 | 0.579589 |
Target: 5'- -cGUGCUCGGgcUGGCaCUGCuCGCGUg -3' miRNA: 3'- acCACGAGCUguACCG-GACG-GUGUAg -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 13731 | 0.68 | 0.558083 |
Target: 5'- aGGUGC-CGugAUGGCCcgcgagauaUGCCAa--- -3' miRNA: 3'- aCCACGaGCugUACCGG---------ACGGUguag -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 8361 | 0.69 | 0.505455 |
Target: 5'- cGGUaucGCUCGGCGgcGGCCU-CgGCAUCg -3' miRNA: 3'- aCCA---CGAGCUGUa-CCGGAcGgUGUAG- -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 46676 | 0.7 | 0.426177 |
Target: 5'- cGGcgaGCUCGGCcUGaGCCgccGCCGCGUCc -3' miRNA: 3'- aCCa--CGAGCUGuAC-CGGa--CGGUGUAG- -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 56091 | 0.7 | 0.41679 |
Target: 5'- cGGUGCUCGuCggGGCCa-CCGCGUg -3' miRNA: 3'- aCCACGAGCuGuaCCGGacGGUGUAg -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 50683 | 0.71 | 0.389403 |
Target: 5'- cGGUGC-CGACGaGGCgCUGCUcguCGUCg -3' miRNA: 3'- aCCACGaGCUGUaCCG-GACGGu--GUAG- -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 28642 | 0.71 | 0.38054 |
Target: 5'- cGGUGC-CGACAccGCCgagGCCGcCGUCa -3' miRNA: 3'- aCCACGaGCUGUacCGGa--CGGU-GUAG- -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 30147 | 0.71 | 0.371812 |
Target: 5'- cGGgcaGCUCGACcgGGCCgccuacaggccGCCACAg- -3' miRNA: 3'- aCCa--CGAGCUGuaCCGGa----------CGGUGUag -5' |
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23301 | 3' | -56.6 | NC_005259.1 | + | 18755 | 1.1 | 0.000663 |
Target: 5'- gUGGUGCUCGACAUGGCCUGCCACAUCg -3' miRNA: 3'- -ACCACGAGCUGUACCGGACGGUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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