Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23301 | 5' | -61.3 | NC_005259.1 | + | 43954 | 0.7 | 0.281642 |
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Target: 5'- -cGGCGaugGGCGGGGcCGCCGCcugACCg -3' miRNA: 3'- guUCGCca-CCGUCCCuGCGGCG---UGGg -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 21390 | 0.7 | 0.249583 |
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Target: 5'- gAGGCGGUGcaacgcGCAuGGGACGCCcGUagACCUg -3' miRNA: 3'- gUUCGCCAC------CGU-CCCUGCGG-CG--UGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 32589 | 0.7 | 0.249583 |
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Target: 5'- -cAGCGGUuuacgcgcGuGCA-GGACGCCGaCACCCu -3' miRNA: 3'- guUCGCCA--------C-CGUcCCUGCGGC-GUGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 48050 | 0.71 | 0.226156 |
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Target: 5'- ---cCGGUGGCcacggcGGGuagcgcCGCCGCGCCCu -3' miRNA: 3'- guucGCCACCGu-----CCCu-----GCGGCGUGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 60425 | 0.71 | 0.226156 |
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Target: 5'- -cGGgGGUGGCccacGGGGCgGCCaCGCCCg -3' miRNA: 3'- guUCgCCACCGu---CCCUG-CGGcGUGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 6199 | 0.72 | 0.199521 |
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Target: 5'- uGAGCaGcGUGaGCGGGGugagcCGCCGCGCCg -3' miRNA: 3'- gUUCG-C-CAC-CGUCCCu----GCGGCGUGGg -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 51642 | 0.72 | 0.189654 |
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Target: 5'- gGAGCGGUaGGCGaacGGGuuGCCGCGCg- -3' miRNA: 3'- gUUCGCCA-CCGU---CCCugCGGCGUGgg -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 35672 | 0.76 | 0.098433 |
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Target: 5'- --cGCGGUGGUGGugcacagcGACGCCGUGCCCg -3' miRNA: 3'- guuCGCCACCGUCc-------CUGCGGCGUGGG- -5' |
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| 23301 | 5' | -61.3 | NC_005259.1 | + | 18789 | 1.1 | 0.000295 |
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Target: 5'- gCAAGCGGUGGCAGGGACGCCGCACCCu -3' miRNA: 3'- -GUUCGCCACCGUCCCUGCGGCGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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