Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23305 | 3' | -53.9 | NC_005259.1 | + | 3313 | 0.66 | 0.824623 |
Target: 5'- aGCGCuGCGucgacgugaGCGcCGGGCAgcUCUGCg -3' miRNA: 3'- gUGUGuCGC---------UGCcGCCCGUaaAGAUG- -5' |
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23305 | 3' | -53.9 | NC_005259.1 | + | 27380 | 0.66 | 0.796303 |
Target: 5'- gGCACGGUGAcCGGC-GGCGgugUCgGCa -3' miRNA: 3'- gUGUGUCGCU-GCCGcCCGUaa-AGaUG- -5' |
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23305 | 3' | -53.9 | NC_005259.1 | + | 45194 | 0.67 | 0.786505 |
Target: 5'- aGCGCGGUGAUGaCGGGCGagUUCg-- -3' miRNA: 3'- gUGUGUCGCUGCcGCCCGUa-AAGaug -5' |
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23305 | 3' | -53.9 | NC_005259.1 | + | 13206 | 0.68 | 0.724729 |
Target: 5'- aGCACAGCGgggccaGCGGUGGGCc--UCg-- -3' miRNA: 3'- gUGUGUCGC------UGCCGCCCGuaaAGaug -5' |
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23305 | 3' | -53.9 | NC_005259.1 | + | 58796 | 0.68 | 0.724729 |
Target: 5'- gAgGCGGUGAuugccuCGGCGGGCucgcgCUGCu -3' miRNA: 3'- gUgUGUCGCU------GCCGCCCGuaaa-GAUG- -5' |
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23305 | 3' | -53.9 | NC_005259.1 | + | 64904 | 0.68 | 0.714042 |
Target: 5'- gACACgAGCGACGGCGaGCGUgaggaugACg -3' miRNA: 3'- gUGUG-UCGCUGCCGCcCGUAaaga---UG- -5' |
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23305 | 3' | -53.9 | NC_005259.1 | + | 3990 | 0.7 | 0.615546 |
Target: 5'- aGCAC-GCGAUGGCGGGCcgcgUCc-- -3' miRNA: 3'- gUGUGuCGCUGCCGCCCGuaa-AGaug -5' |
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23305 | 3' | -53.9 | NC_005259.1 | + | 54016 | 0.7 | 0.582608 |
Target: 5'- cCAC-CAGCGACGcuCGGGCGcggugcggUCUGCg -3' miRNA: 3'- -GUGuGUCGCUGCc-GCCCGUaa------AGAUG- -5' |
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23305 | 3' | -53.9 | NC_005259.1 | + | 5643 | 0.71 | 0.518056 |
Target: 5'- uGCcCGGCGAggaCGGCGGGCg---CUACa -3' miRNA: 3'- gUGuGUCGCU---GCCGCCCGuaaaGAUG- -5' |
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23305 | 3' | -53.9 | NC_005259.1 | + | 200 | 0.72 | 0.497147 |
Target: 5'- gGCGCGuggcGCGACGGCaagcaguucGGGCAgugCUACg -3' miRNA: 3'- gUGUGU----CGCUGCCG---------CCCGUaaaGAUG- -5' |
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23305 | 3' | -53.9 | NC_005259.1 | + | 41491 | 0.72 | 0.476627 |
Target: 5'- gCACGaccuCGGCGGUGGCGGGCAgaccguggaUCUGCa -3' miRNA: 3'- -GUGU----GUCGCUGCCGCCCGUaa-------AGAUG- -5' |
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23305 | 3' | -53.9 | NC_005259.1 | + | 3094 | 0.79 | 0.182232 |
Target: 5'- cCGCGCAGCGAUGaGCGGGCGgccaUUGCg -3' miRNA: 3'- -GUGUGUCGCUGC-CGCCCGUaaa-GAUG- -5' |
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23305 | 3' | -53.9 | NC_005259.1 | + | 17532 | 1.09 | 0.001613 |
Target: 5'- gCACACAGCGACGGCGGGCAUUUCUACu -3' miRNA: 3'- -GUGUGUCGCUGCCGCCCGUAAAGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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