Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23308 | 3' | -61.7 | NC_005259.1 | + | 44497 | 0.66 | 0.474597 |
Target: 5'- uGACGUAG---ACCGGGGUGCCCu-- -3' miRNA: 3'- cCUGCGUCgggUGGCUCCACGGGcag -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 37229 | 0.66 | 0.474597 |
Target: 5'- gGGGCGCuGGCCCguGCCGGauuGGcUGCCgcCGUUg -3' miRNA: 3'- -CCUGCG-UCGGG--UGGCU---CC-ACGG--GCAG- -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 9124 | 0.66 | 0.474597 |
Target: 5'- -cACGC-GCCCcgguacgggcaGCCGugcucGUGCCCGUCg -3' miRNA: 3'- ccUGCGuCGGG-----------UGGCuc---CACGGGCAG- -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 41013 | 0.66 | 0.465124 |
Target: 5'- cGGGC-CGGUCCACaguGuGGUGCCgcUGUCa -3' miRNA: 3'- -CCUGcGUCGGGUGg--CuCCACGG--GCAG- -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 39902 | 0.66 | 0.465124 |
Target: 5'- cGcCGCcGCCCGCCucgauccgucGAGG-GCCgCGUCg -3' miRNA: 3'- cCuGCGuCGGGUGG----------CUCCaCGG-GCAG- -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 20399 | 0.66 | 0.465124 |
Target: 5'- cGACGUAGCaagggCAUCGAGGUcuaCCCGgUCa -3' miRNA: 3'- cCUGCGUCGg----GUGGCUCCAc--GGGC-AG- -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 290 | 0.66 | 0.45482 |
Target: 5'- -uACGCGGCCUcgcgcugaucucgACCGGGG-GUgCGUCc -3' miRNA: 3'- ccUGCGUCGGG-------------UGGCUCCaCGgGCAG- -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 55030 | 0.66 | 0.446481 |
Target: 5'- cGGCaCGGgCUGCCGAGGUGCuuGcCu -3' miRNA: 3'- cCUGcGUCgGGUGGCUCCACGggCaG- -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 62196 | 0.66 | 0.446481 |
Target: 5'- -cACGCAcacggucaccGCUCACaCGAGGUGCCuCGa- -3' miRNA: 3'- ccUGCGU----------CGGGUG-GCUCCACGG-GCag -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 13464 | 0.66 | 0.437316 |
Target: 5'- cGGCGCugguGCUCACCGcGcUGCCCGa- -3' miRNA: 3'- cCUGCGu---CGGGUGGCuCcACGGGCag -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 12542 | 0.66 | 0.428261 |
Target: 5'- -aGCG-AGCCCcacggcauuGCCGGGGUgGCCCGcCa -3' miRNA: 3'- ccUGCgUCGGG---------UGGCUCCA-CGGGCaG- -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 60425 | 0.67 | 0.419317 |
Target: 5'- cGGGgGUGGCCCACgGGGcGgccacGCCCGa- -3' miRNA: 3'- -CCUgCGUCGGGUGgCUC-Ca----CGGGCag -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 41402 | 0.67 | 0.410487 |
Target: 5'- uGGCGCG--CCGCuCGGGGUGCCCa-- -3' miRNA: 3'- cCUGCGUcgGGUG-GCUCCACGGGcag -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 34872 | 0.67 | 0.401774 |
Target: 5'- cGuCGguGCCgGaCCGGGGUGCCuCGg- -3' miRNA: 3'- cCuGCguCGGgU-GGCUCCACGG-GCag -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 60575 | 0.67 | 0.401774 |
Target: 5'- cGGucAgGCGGCCCACgGcgagucGGGUG-CCGUCa -3' miRNA: 3'- -CC--UgCGUCGGGUGgC------UCCACgGGCAG- -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 17456 | 0.67 | 0.39318 |
Target: 5'- cGAUGUGccaccGCCCACCGuGGUgacGCCCGg- -3' miRNA: 3'- cCUGCGU-----CGGGUGGCuCCA---CGGGCag -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 68558 | 0.67 | 0.39318 |
Target: 5'- gGGugGUugAGCCaACCGAGGUGgacCUCGUa -3' miRNA: 3'- -CCugCG--UCGGgUGGCUCCAC---GGGCAg -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 739 | 0.67 | 0.39318 |
Target: 5'- cGGCGguGCCUacGCCGccGGUGCCuaccgCGUCc -3' miRNA: 3'- cCUGCguCGGG--UGGCu-CCACGG-----GCAG- -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 67085 | 0.67 | 0.383864 |
Target: 5'- uGACGCcgaccuuGGCCCAuugguCCGAGGUguacuGCCCGc- -3' miRNA: 3'- cCUGCG-------UCGGGU-----GGCUCCA-----CGGGCag -5' |
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23308 | 3' | -61.7 | NC_005259.1 | + | 68339 | 0.67 | 0.376353 |
Target: 5'- gGGGCucaGCGGCUCAUCGGGGUaGCUCaUCc -3' miRNA: 3'- -CCUG---CGUCGGGUGGCUCCA-CGGGcAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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