Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23309 | 5' | -61.9 | NC_005259.1 | + | 46352 | 0.66 | 0.440258 |
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Target: 5'- -gUAGGUucuGGGCCgcGAugGCACcgCCCGGc -3' miRNA: 3'- uaGUCCA---CCCGG--CUugCGUG--GGGCCa -5' |
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| 23309 | 5' | -61.9 | NC_005259.1 | + | 1545 | 0.66 | 0.422016 |
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Target: 5'- cAUCGGGgccgcguucgGGGCggugacgcugUGAGCGCGCCCCa-- -3' miRNA: 3'- -UAGUCCa---------CCCG----------GCUUGCGUGGGGcca -5' |
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| 23309 | 5' | -61.9 | NC_005259.1 | + | 45613 | 0.66 | 0.39466 |
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Target: 5'- cUC-GGUcucgaugcccgccGGGCCGGGCaGCGCgCCGGUg -3' miRNA: 3'- uAGuCCA-------------CCCGGCUUG-CGUGgGGCCA- -5' |
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| 23309 | 5' | -61.9 | NC_005259.1 | + | 58562 | 0.67 | 0.386936 |
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Target: 5'- cUCGGGcgguUGGGCCGGAuCGCGgCgCGGc -3' miRNA: 3'- uAGUCC----ACCCGGCUU-GCGUgGgGCCa -5' |
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| 23309 | 5' | -61.9 | NC_005259.1 | + | 11432 | 0.68 | 0.338047 |
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Target: 5'- -aCGGGgaugcuUGGGCCGcuCGCuGCCgCCGGUg -3' miRNA: 3'- uaGUCC------ACCCGGCuuGCG-UGG-GGCCA- -5' |
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| 23309 | 5' | -61.9 | NC_005259.1 | + | 4513 | 0.69 | 0.286888 |
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Target: 5'- -cCGGGagUGGcGCUGcGCgGCACCCCGGUc -3' miRNA: 3'- uaGUCC--ACC-CGGCuUG-CGUGGGGCCA- -5' |
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| 23309 | 5' | -61.9 | NC_005259.1 | + | 7931 | 0.7 | 0.248058 |
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Target: 5'- gGUCucGUGGGCCuucuuGCGCGCCCaCGGc -3' miRNA: 3'- -UAGucCACCCGGcu---UGCGUGGG-GCCa -5' |
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| 23309 | 5' | -61.9 | NC_005259.1 | + | 17839 | 0.76 | 0.085046 |
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Target: 5'- -aCGGGUGGGCCGAgGC-CACCCCGuGg -3' miRNA: 3'- uaGUCCACCCGGCU-UGcGUGGGGC-Ca -5' |
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| 23309 | 5' | -61.9 | NC_005259.1 | + | 16222 | 1.05 | 0.000533 |
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Target: 5'- gAUCAGGUGGGCCGAACGCACCCCGGUg -3' miRNA: 3'- -UAGUCCACCCGGCUUGCGUGGGGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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