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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2331 | 5' | -48.4 | NC_001416.1 | + | 3651 | 1.06 | 0.00594 |
Target: 5'- uCUGCUCCAUCACGCUGUAAAACACAUu -3' miRNA: 3'- -GACGAGGUAGUGCGACAUUUUGUGUA- -5' |
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2331 | 5' | -48.4 | NC_001416.1 | + | 3610 | 0.72 | 0.673218 |
Target: 5'- aUGCUCgA-CACGCUGcAGAACACGc -3' miRNA: 3'- gACGAGgUaGUGCGACaUUUUGUGUa -5' |
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2331 | 5' | -48.4 | NC_001416.1 | + | 2474 | 0.69 | 0.827666 |
Target: 5'- aCUGCUUCGUUuauGCGCUGgcGGcgcuGCGCAUc -3' miRNA: 3'- -GACGAGGUAG---UGCGACauUU----UGUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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