Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23310 | 3' | -57 | NC_005259.1 | + | 55307 | 0.66 | 0.702606 |
Target: 5'- gGCcGCGUGCgACgaccaCAGGGgUGGUCUUGg -3' miRNA: 3'- -CGaCGCACGgUG-----GUUCCgGCUAGAGC- -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 52385 | 0.66 | 0.698399 |
Target: 5'- gGCUGCGcagccugccucggGCCAUCGAGGUgUGGgcgCUCGa -3' miRNA: 3'- -CGACGCa------------CGGUGGUUCCG-GCUa--GAGC- -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 16370 | 0.66 | 0.68148 |
Target: 5'- -gUGCGUGCCGCUgc-GCCGAcgagCUCc -3' miRNA: 3'- cgACGCACGGUGGuucCGGCUa---GAGc -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 58582 | 0.66 | 0.670846 |
Target: 5'- cGCgGCGcgGCUGCC-GGGCCgGAUCUUu -3' miRNA: 3'- -CGaCGCa-CGGUGGuUCCGG-CUAGAGc -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 61433 | 0.66 | 0.660176 |
Target: 5'- cUUGCGgGCgCGCC--GGUCGAUCUCa -3' miRNA: 3'- cGACGCaCG-GUGGuuCCGGCUAGAGc -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 36432 | 0.67 | 0.649482 |
Target: 5'- uCUGCGcagcGUCGCCGAGGUCGAccgCgUCGa -3' miRNA: 3'- cGACGCa---CGGUGGUUCCGGCUa--G-AGC- -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 53443 | 0.67 | 0.649482 |
Target: 5'- aCUGagcagGUGCCACCAcggcGGCUGG-CUCGu -3' miRNA: 3'- cGACg----CACGGUGGUu---CCGGCUaGAGC- -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 66670 | 0.67 | 0.649482 |
Target: 5'- uGCUGCGaugcucaucgUGCCGCCcauGAGGUCGAaaauggUgUCGu -3' miRNA: 3'- -CGACGC----------ACGGUGG---UUCCGGCU------AgAGC- -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 61256 | 0.67 | 0.638773 |
Target: 5'- gGgUGCGagcaUGCgACCGucGCUGGUCUCGa -3' miRNA: 3'- -CgACGC----ACGgUGGUucCGGCUAGAGC- -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 22787 | 0.67 | 0.628058 |
Target: 5'- aCUGUgcagGUGCuCACCGAGgguGCCGAUUUCc -3' miRNA: 3'- cGACG----CACG-GUGGUUC---CGGCUAGAGc -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 32994 | 0.67 | 0.628058 |
Target: 5'- --aGgGUGCCGCCAcGcCCGAUgUCGg -3' miRNA: 3'- cgaCgCACGGUGGUuCcGGCUAgAGC- -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 29933 | 0.67 | 0.617346 |
Target: 5'- --cGCGUGgCCGCCAcgcGGCcCGGUCgCGg -3' miRNA: 3'- cgaCGCAC-GGUGGUu--CCG-GCUAGaGC- -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 1312 | 0.67 | 0.606647 |
Target: 5'- cGCgGuCGUGagCGCCAuGGUCGAUUUCGa -3' miRNA: 3'- -CGaC-GCACg-GUGGUuCCGGCUAGAGC- -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 8656 | 0.67 | 0.606647 |
Target: 5'- gGCUGUGgucggGCUGCCAcgcgggcaacgGGGCCGc-CUCGa -3' miRNA: 3'- -CGACGCa----CGGUGGU-----------UCCGGCuaGAGC- -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 33105 | 0.67 | 0.606647 |
Target: 5'- cGCgaGCGUGCCugUAGGGCCa------ -3' miRNA: 3'- -CGa-CGCACGGugGUUCCGGcuagagc -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 6931 | 0.67 | 0.595968 |
Target: 5'- --cGCc-GCCGCCuuGAGGCCGAgCUCGc -3' miRNA: 3'- cgaCGcaCGGUGG--UUCCGGCUaGAGC- -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 43881 | 0.67 | 0.594902 |
Target: 5'- gGCaGCGuUGCCGCCGAcauugcccgcgccGGCUGAUCg-- -3' miRNA: 3'- -CGaCGC-ACGGUGGUU-------------CCGGCUAGagc -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 34554 | 0.68 | 0.543167 |
Target: 5'- --aGCuUGCCGUCGGGGCCGAcggcgagguUCUCGg -3' miRNA: 3'- cgaCGcACGGUGGUUCCGGCU---------AGAGC- -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 12029 | 0.68 | 0.532776 |
Target: 5'- cGgUGCGggcgGCaaccgguacuCACCGAGGCCGAgCUCu -3' miRNA: 3'- -CgACGCa---CG----------GUGGUUCCGGCUaGAGc -5' |
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23310 | 3' | -57 | NC_005259.1 | + | 10250 | 0.69 | 0.491992 |
Target: 5'- gGCgGUGUGCUugAUgAgcGGGCCGGUCUCa -3' miRNA: 3'- -CGaCGCACGG--UGgU--UCCGGCUAGAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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