Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23315 | 3' | -60.7 | NC_005259.1 | + | 58938 | 0.67 | 0.439292 |
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Target: 5'- gGGCGCGGUGGcagcgucGGCCUugGccaGCAg -3' miRNA: 3'- gUCGUGCCGCCa------CCGGAugCacgCGU- -5' |
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| 23315 | 3' | -60.7 | NC_005259.1 | + | 10452 | 0.67 | 0.439292 |
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Target: 5'- uGGCucgACGGCGGUGuCCUGCGcaGCGgGa -3' miRNA: 3'- gUCG---UGCCGCCACcGGAUGCa-CGCgU- -5' |
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| 23315 | 3' | -60.7 | NC_005259.1 | + | 29128 | 0.66 | 0.44864 |
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Target: 5'- gAGCGCGGCGGUauucGCCU-CGcucaccGCGCu -3' miRNA: 3'- gUCGUGCCGCCAc---CGGAuGCa-----CGCGu -5' |
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| 23315 | 3' | -60.7 | NC_005259.1 | + | 13205 | 0.66 | 0.467658 |
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Target: 5'- aAGCACaGCGG-GGCCaGCgGUGgGCc -3' miRNA: 3'- gUCGUGcCGCCaCCGGaUG-CACgCGu -5' |
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| 23315 | 3' | -60.7 | NC_005259.1 | + | 60421 | 0.66 | 0.477322 |
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Target: 5'- -uGCuCGGgGGUGGCCcACG-GgGCGg -3' miRNA: 3'- guCGuGCCgCCACCGGaUGCaCgCGU- -5' |
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| 23315 | 3' | -60.7 | NC_005259.1 | + | 45481 | 0.66 | 0.487083 |
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Target: 5'- gAGCuucUGGCcgcuGGUGGCCUugGU-CGCu -3' miRNA: 3'- gUCGu--GCCG----CCACCGGAugCAcGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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