Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23315 | 3' | -60.7 | NC_005259.1 | + | 7177 | 0.69 | 0.31373 |
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Target: 5'- gGGCAcCGGCGaGUgcccGGCCUGCG-GaCGCAc -3' miRNA: 3'- gUCGU-GCCGC-CA----CCGGAUGCaC-GCGU- -5' |
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| 23315 | 3' | -60.7 | NC_005259.1 | + | 10244 | 0.69 | 0.299193 |
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Target: 5'- gAGCACGGCGGUGuGCUUgAUGaGCGg- -3' miRNA: 3'- gUCGUGCCGCCAC-CGGA-UGCaCGCgu -5' |
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| 23315 | 3' | -60.7 | NC_005259.1 | + | 62750 | 0.69 | 0.29848 |
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Target: 5'- gCAGUacGCGGUGGUacucgugGGCCggaACGUcGCGCAg -3' miRNA: 3'- -GUCG--UGCCGCCA-------CCGGa--UGCA-CGCGU- -5' |
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| 23315 | 3' | -60.7 | NC_005259.1 | + | 57314 | 0.7 | 0.292122 |
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Target: 5'- gAGCAcCGGCGGUgucgguguGGUC-AUGUGCGCu -3' miRNA: 3'- gUCGU-GCCGCCA--------CCGGaUGCACGCGu -5' |
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| 23315 | 3' | -60.7 | NC_005259.1 | + | 37759 | 0.7 | 0.271696 |
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Target: 5'- cCAGCGCGGUGGcc-CCUGCGggcacgaGCGCAu -3' miRNA: 3'- -GUCGUGCCGCCaccGGAUGCa------CGCGU- -5' |
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| 23315 | 3' | -60.7 | NC_005259.1 | + | 14070 | 1.08 | 0.000449 |
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Target: 5'- cCAGCACGGCGGUGGCCUACGUGCGCAg -3' miRNA: 3'- -GUCGUGCCGCCACCGGAUGCACGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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