Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23317 | 5' | -52.8 | NC_005259.1 | + | 25108 | 0.66 | 0.872047 |
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Target: 5'- -aGCAAGGGcaAGGUCgACCCCGc---- -3' miRNA: 3'- agUGUUCCC--UCUAGgUGGGGUacaag -5' |
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| 23317 | 5' | -52.8 | NC_005259.1 | + | 29324 | 0.66 | 0.863985 |
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Target: 5'- aCACcaaGGAGAUaCACCCCAUGa-- -3' miRNA: 3'- aGUGuucCCUCUAgGUGGGGUACaag -5' |
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| 23317 | 5' | -52.8 | NC_005259.1 | + | 25028 | 0.66 | 0.863985 |
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Target: 5'- aCACAAaGGAGAU-CACCaCCAUGg-- -3' miRNA: 3'- aGUGUUcCCUCUAgGUGG-GGUACaag -5' |
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| 23317 | 5' | -52.8 | NC_005259.1 | + | 17838 | 0.67 | 0.847132 |
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Target: 5'- -gACGGGuGGGccGAggCCACCCCGUGgUCa -3' miRNA: 3'- agUGUUC-CCU--CUa-GGUGGGGUACaAG- -5' |
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| 23317 | 5' | -52.8 | NC_005259.1 | + | 1147 | 0.67 | 0.838357 |
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Target: 5'- -aACAGGaaGGA-CCACCaCCAUGUUCg -3' miRNA: 3'- agUGUUCccUCUaGGUGG-GGUACAAG- -5' |
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| 23317 | 5' | -52.8 | NC_005259.1 | + | 13364 | 1.11 | 0.001473 |
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Target: 5'- aUCACAAGGGAGAUCCACCCCAUGUUCa -3' miRNA: 3'- -AGUGUUCCCUCUAGGUGGGGUACAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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